Re: [ccp4bb] Challenging Mol.Rep. Problem

2014-12-09 Thread Artem Evdokimov
Generally fab geometry presents problems for mr due to variable interdomain angle. Assuming your sg is correct and this is not a twin, i would try searching at 4 or even 5 a resolution. I would not reject the chance that you have high solvent content, so you might have fewer molecules in au than

Re: [ccp4bb] Challenging Mol.Rep. Problem

2014-12-09 Thread Artem Evdokimov
Thank you cell phone typing for the inadvertent prophanity. Sorry! On Dec 9, 2014 1:00 PM, Artem Evdokimov artem.evdoki...@gmail.com wrote: Generally fab geometry presents problems for mr due to variable interdomain angle. Assuming your sg is correct and this is not a twin, i would try

Re: [ccp4bb] Challenging Mol.Rep. Problem

2014-12-09 Thread Eleanor Dodson
Try othercell to look for other indexing - C2 is a possible alternate SG to H32. And have you run pointless to check on symmetry operators? You certainly have 4 independent 3 fold rotations which would generate 12 mols per ASU. But first think about higher symmetry SGs - that will make the whole

Re: [ccp4bb] Challenging Mol.Rep. Problem

2014-12-09 Thread Randy Read
Hi, We’ve had good luck with Phaser in placing many copies of proteins or domains, if the model is reasonably good. Because of the variable elbow angle, you probably want to place the domains separately. However, as soon as you find one convincing assembly you can switch to searching for the