Generally fab geometry presents problems for mr due to variable interdomain
angle. Assuming your sg is correct and this is not a twin, i would try
searching at 4 or even 5 a resolution. I would not reject the chance that
you have high solvent content, so you might have fewer molecules in au than
Thank you cell phone typing for the inadvertent prophanity. Sorry!
On Dec 9, 2014 1:00 PM, Artem Evdokimov artem.evdoki...@gmail.com wrote:
Generally fab geometry presents problems for mr due to variable
interdomain angle. Assuming your sg is correct and this is not a twin, i
would try
Try othercell to look for other indexing - C2 is a possible alternate SG to
H32.
And have you run pointless to check on symmetry operators?
You certainly have 4 independent 3 fold rotations which would generate 12
mols per ASU.
But first think about higher symmetry SGs - that will make the whole
Hi,
We’ve had good luck with Phaser in placing many copies of proteins or domains,
if the model is reasonably good. Because of the variable elbow angle, you
probably want to place the domains separately. However, as soon as you find
one convincing assembly you can switch to searching for the