You don't say whether you have any indication of non cryst translation or
likely dimer NC axes? The self rotation can help sometimes to select likely
pairings - for instance if one (or both) domain(s) is forming a dimer.
Eleanor
On 26 Oct 2012, at 13:43, Bosch, Juergen wrote:
> We had rece
Hello Jose,
Depending on what data integration program you used, trying XDS may help
you out a little with spot overlap.
Example #3 in my rather out-of-date page:
http://xray0.princeton.edu/~phil/Facility/Guides/MolecularReplacement.html
illustrates how you could find 8 domains, especially if
I had a similar issue (8 chains in a large ASU, 2.4 A resolution, 29%
homology, possibly--and ultimately confirmed--twinned data). Here is how
I solved my structure (not pretty!):
1. Phaser was used to find an initial placement for the chains using
(what turned out to be) the twin maps. This
We had recently a similar case.
Indexed in P2x2x2x but truly was P21 with variable amount of twin fraction
depending on the dataset & beamline between 9% and 40%.
We were able to solve it using phenix.ensembler using all available known
structures plus our various homology models.
We still have n
Hi all,
I am dealing with a molecular replacement problem for a 60KDa protein composed
of 2 rigid domains joined by a flexible linker which can move relative to each
other. Sequence identity for my best model is 46% evenly spread, so in
principle this should be a tractable problem.
Then the
