it should be.
Best regards,
Herman
-Original Message-
From: CCP4 bulletin board [mailto:ccp...@jiscmail.ac.uk] On Behalf Of
Damian Ekiert
Sent: Wednesday, December 23, 2009 2:52 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] Coot pudding? (a.k.a N-linked carbohydrate
addition)
Pierre
2:52 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] Coot pudding? (a.k.a N-linked carbohydrate
addition)
Pierre,
For what its worth, I just tried using Real Space Refine Zone on
something I am working on now (2.8A resolution, in coot 0.5.2, build
1691). With the LINKR record generated
Summary of this thread:
FUC-MAN-NAG-NAG-NAG
Merry Flying-Spaghetti-Monster-mas (*) everybody!
--Gerard
(* http://www.venganza.org/)
**
Gerard J. Kleywegt
Dept. of Cell Molecular Biology
: Re: [ccp4bb] Coot pudding? (a.k.a N-linked carbohydrate addition)
To: CCP4BB@JISCMAIL.AC.UK
Steve,
My general strategy is to start with an ideal glycan (an Asn linked to
NAG-NAG-(MAN)3 ) and superimpose the Asn on the residue from my protein.
Then you can move the whole glycan as a rigid body
Dec 2009 17:48:31 -0800
From: dceki...@scripps.edu
Subject: Re: [ccp4bb] Coot pudding? (a.k.a N-linked carbohydrate addition)
To: CCP4BB@JISCMAIL.AC.UK
Steve,
My general strategy is to start with an ideal glycan (an Asn linked to
NAG-NAG-(MAN)3 ) and superimpose the Asn on the residue from my
atoms
when needed ;)
Cheers,
Robbie Joosten
Date: Mon, 21 Dec 2009 17:48:31 -0800
From: dceki...@scripps.edu
Subject: Re: [ccp4bb] Coot pudding? (a.k.a N-linked carbohydrate
addition)
To: CCP4BB@JISCMAIL.AC.UK
Steve,
My general strategy is to start
I am also having problems with the branching.
The fucose linked to the first NAG being linked to the second NAG of the
carbohydrate chain.
So one has a linear chain NAG-FUC-NAG-MAN-MAN-MAN
instead of the FUC being a branch out from the first NAG. i.e. :
NAG-NAG-BMA-BMA
||
FUC MAN
needed ;)
Cheers,
Robbie Joosten
Date: Mon, 21 Dec 2009 17:48:31 -0800
From: dceki...@scripps.edu
Subject: Re: [ccp4bb] Coot pudding? (a.k.a N-linked carbohydrate addition)
To: CCP4BB@JISCMAIL.AC.UK
Steve,
My general strategy
: Mon, 21 Dec 2009 17:48:31 -0800
From: dceki...@scripps.edu
Subject: Re: [ccp4bb] Coot pudding? (a.k.a N-linked carbohydrate addition)
To: CCP4BB@JISCMAIL.AC.UK
Steve,
My general strategy is to start with an ideal glycan (an Asn linked to
NAG-NAG-(MAN)3 ) and superimpose the Asn on the residue
Steve,
My general strategy is to start with an ideal glycan (an Asn linked to
NAG-NAG-(MAN)3 ) and superimpose the Asn on the residue from my protein.
Then you can move the whole glycan as a rigid body until the Asn and
first NAG are roughly positioned. Then you can tweak any sugars further
Date: Mon, 21 Dec 2009 17:48:31 -0800
From: dceki...@scripps.edu
Subject: Re: [ccp4bb] Coot pudding? (a.k.a N-linked carbohydrate addition)
To: CCP4BB@JISCMAIL.AC.UK
Steve,
My general strategy is to start with an ideal glycan (an Asn linked to
NAG-NAG-(MAN)3 ) and superimpose
Hi everyone-
I was searching for some information on what might be the best way to
add N-linked sugars in coot, and Google has let me down. Searching
adding sugars in coot returns a very nice recipe for Coot Pudding.
Recipe for Coot Pudding - American Coots
http://www.beakycoot.com/pudding.html
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