Hello everyone
I am working on a 5'-nucleotidase (Mg as a cofactor) and have a decent
structure of the same at 2A resolution..While doing its biochemical
characterization i found it hydrolyzing TMP: Thymine-5'-monophosphate at
much higher rate than CMP Cytosine-5'-monophosphate (40 fold
Hi Faisal,
Lots of enzymes aren't too picky when it comes to nucleotide cofactors -
depending on their physiological role, they may or may not select for high
specificity. I'd encourage you to think about the opposite question - why
are some enzymes highly specific for particular nucleosides?
