Re: [ccp4bb] Formulation of my own mother liquor

2014-11-24 Thread Petri Kursula
Well I think some companies make the screens from stocks, the others adjust the pH of the final mixture, so you might want to check the small print for the screen you used in the first placeā€¦ But when optimizing with your own solutions, you would in general take the easy way out - make good sto

Re: [ccp4bb] Formulation of my own mother liquor

2014-11-21 Thread David Schuller
The most important thing is that you do it the same way every time. But you might also put some thought into flexibility, in case you need to explore cryoprotectants, etc. On 11/21/14 11:48, amro selem wrote: Dear All, first i wish you nice weekend, then i wanna ask about formulation of my ow

Re: [ccp4bb] Formulation of my own mother liquor

2014-11-21 Thread Janet Newman
of amro selem [03391c09f749-dmarc-requ...@jiscmail.ac.uk] Sent: 22 November 2014 03:48 To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] Formulation of my own mother liquor Dear All, first i wish you nice weekend, then i wanna ask about formulation of my own mother liquor, i want to optimize a

[ccp4bb] Formulation of my own mother liquor

2014-11-21 Thread amro selem
Dear All,first i wish you nice weekend, then i wanna ask about formulation of my own mother liquor, i want to optimize a condition containingĀ 0.2m MgCl2, 0.1m Tris , PH 8; and 20% PEG6K as pricipitant. (PACT)the question is , should i make a stock solution from every ingredient by dissolving it