Re: [ccp4bb] How can improve diffraction quality

2011-10-20 Thread James Holton
An excellent review of this subject was published not long ago: http://dx.doi.org/10.1107/S0907444905032130 It is even open access! But, in general the trick to improving diffraction is to get your molecules clean, get them to all adopt the same conformation, and then sit still. Adding a

[ccp4bb] How can improve diffraction quality

2011-10-18 Thread Afshan Begum
 Dear ccp4 user I am facing one crucial problem regarding diffraction. Actually the size of my crystal is good enough 0.5mm but it  was diffracted only 4 A. The conditions of crystallization recipes are 4.5% PEG 1000, 0.1M Tris and 25mM Na citrate. I really need your suggestions regarding 

Re: [ccp4bb] How can improve diffraction quality

2011-10-18 Thread elizabeth . duke
Diamond Light Source Harwell Science and Innovation Campus Chilton OX11 0DE UK Tel. 01235 778057 Mob. 07920 138148 From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Afshan Begum Sent: 18 October 2011 12:45 To: ccp4bb Subject: [ccp4bb] How can improve diffraction quality Dear ccp4

Re: [ccp4bb] How can improve diffraction quality

2011-10-18 Thread Charles Allerston
To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] How can improve diffraction quality Dear ccp4 user I am facing one crucial problem regarding diffraction. Actually the size of my crystal is good enough 0.5mm but it was diffracted only 4 A. The conditions of crystallization recipes are 4.5% PEG 1000

Re: [ccp4bb] How can improve diffraction quality

2011-10-18 Thread vandana kukshal
] On Behalf Of Afshan Begum [afshan...@yahoo.com] Sent: 18 October 2011 12:45 To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] How can improve diffraction quality Dear ccp4 user I am facing one crucial problem regarding diffraction. Actually the size of my crystal is good enough 0.5mm