An excellent review of this subject was published not long ago:
http://dx.doi.org/10.1107/S0907444905032130
It is even open access!
But, in general the trick to improving diffraction is to get your
molecules clean, get them to all adopt the same conformation, and then
sit still. Adding a
Dear ccp4 user
I am facing one crucial problem regarding diffraction. Actually the size of my
crystal is good enough 0.5mm but it was diffracted only 4 A.
The conditions of crystallization recipes are 4.5% PEG 1000, 0.1M Tris and 25mM
Na citrate. I really need your suggestions regarding
Diamond Light Source
Harwell Science and Innovation Campus
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OX11 0DE
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Tel. 01235 778057
Mob. 07920 138148
From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Afshan
Begum
Sent: 18 October 2011 12:45
To: ccp4bb
Subject: [ccp4bb] How can improve diffraction quality
Dear ccp4
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] How can improve diffraction quality
Dear ccp4 user
I am facing one crucial problem regarding diffraction. Actually the size of my
crystal is good enough 0.5mm but it was diffracted only 4 A.
The conditions of crystallization recipes are 4.5% PEG 1000
] On Behalf Of Afshan
Begum [afshan...@yahoo.com]
Sent: 18 October 2011 12:45
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] How can improve diffraction quality
Dear ccp4 user
I am facing one crucial problem regarding diffraction. Actually the size of
my crystal is good enough 0.5mm