Hi,
If there is a hinge motion between the two domains, then allowing for this will
give you a much better starting model. As Klaus suggested, you may just be
able to do rigid body refinement of the two domains. However, it is also
possible to place the two domains separately in Phaser, by
Thank you very much for kind suggestions and comment.
With kind regards
Prem
On Mon, Dec 2, 2013 at 5:41 PM, Randy Read rj...@cam.ac.uk wrote:
Hi,
If there is a hinge motion between the two domains, then allowing for this
will give you a much better starting model. As Klaus suggested, you
Dear All,
The density obtained after molecular replacement using phaser at 2.5
Angstrom and then used buccneer for autobuilding of the model. I am not
getting reasonable R value (it is 38.5 %) but the figure of merit is 0.629.
As My protein has two domains. So is it possible to fragment the
Dear Prem,
If your protein has 2 domains, it is possible that they their relative
orientation is different in your target compared to the search model. Therefore
searching with the two domains separately can improve your Z-score in Phaser
and give you a better map.
However, you did not tell
