On Fri, 30 Nov 2012 17:27:41 -0500, Sarathy Karunan Partha
sarathyus...@gmail.com wrote:
Dear all,
Here is the XSCALE.LP output after scaling for the izit dye stained
crystak. Sorry for attaching the .INP file.
Sarathy
On Fri, Nov 30, 2012 at 4:19 PM, Sarathy Karunan Partha
Dear Sarathy,
I was going to reply along the same lines as Kay Diederichs just
did. At the Cr K-alpha wavelength, you would have strong absorption
effects which, given the low symmetry of your crystal, would be very
hard to get rid of. We have seen numerous cases where a high anomalous
Dear all,
We did some Izit dye staining to test our crystal (salt or protein) and we
observed that the crystal didn’t take up the dye well. But, showed nice
protein diffraction (home source KCr 2.2909 A) and we collected a dataset
(360 frames, 1o osc, 5 min exposure) on this dye stained
Hi Sarathy,
Are you sure your anomalous scatterers are not the Ca in the
crystallisation buffer? These would also bind to the acidic residues in
your protein, and Ca has a greater f'' (~2.5e compared to less than 1.5e
for S) at the wavelength you used.
Just another possibility - unless you
We've found that high PEG concentration seems to compete with dye binding, so
I'm not surprised your didn't see good uptake.
I doubt the anomalous signal is from the dye ... if you calculate the molar dye
concentration you would need to have significant occupancy in the lattice,
you'll probably
Dear all,
Here is the XSCALE.LP output after scaling for the izit dye stained
crystak. Sorry for attaching the .INP file.
Sarathy
On Fri, Nov 30, 2012 at 4:19 PM, Sarathy Karunan Partha
sarathyus...@gmail.com wrote:
Dear all,
We did some Izit dye staining to test our crystal (salt or
Hi Sarathy,
What does the density modified electron density map after phasing from either
autoSHARP or Phenix? Can the auto building programs build protein chains
into this map?
Cheers,
Scott
On Nov 30, 2012, at 5:27 PM, Sarathy Karunan Partha
@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] Izit dye stained crystal
We've found that high PEG concentration seems to compete with dye
binding, so I'm not surprised your didn't see good uptake.
I doubt the anomalous signal is from the dye ... if you calculate the
molar dye concentration you would need
or
disclosure of the contents of this message is not permitted and may be unlawful.
From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Richard
Gillilan
Sent: Friday, November 30, 2012 5:21 PM
To: CCP4BB@JISCMAIL.AC.UKmailto:CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] Izit dye
