K+ and NH4+ ions precipitate SDS - forming the lovely cheese that makes it
very difficult to load and run those samples.
Sometimes using LDS can help with the potassium issue. Heating the sample
right before loading might help as well, or using a lot larger ratio of
buffer to sample.
of Maryland at Baltimore
725 W. Lombard Street N571
Baltimore
MD 21201
From: CCP4 bulletin board on behalf of Keller, Jacob
Sent: Saturday, March 2, 2019 9:52 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] KCl in SDS-PAGE workarounds?
Dear crystallographers
725 W. Lombard Street N571
Baltimore
MD 21201
From: CCP4 bulletin board on behalf of Keller, Jacob
Sent: Saturday, March 2, 2019 9:52 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] KCl in SDS-PAGE workarounds?
Dear crystallographers,
It has been my experience
Dear crystallographers,
It has been my experience that KCl does nasty things when loading SDS-PAGE
gels. Does anyone have an easy workaround, perhaps TCA precipitation? Ideally
this would be something nicely quantitative yet quick and easy….
Any suggestions appreciated.
All the best,
Jacob
