Re: [ccp4bb] MR for coiled coil structure

.AC.UK Subject: [ccp4bb] MR for coiled coil structure Dear all, We recently acquired a data set (2.0 A, P222) for a coiled coil protein (according to Itasser, QUARK, Robetta, and Phaser). Matthews coefficient indicates 1 copy of protein per ASU. Sequence of the protein is quite novel with no ap

Re: [ccp4bb] MR for coiled coil structure

of Shengyang Jin mailto:jinshengy...@outlook.com>> Sent: 10 July 2019 09:00 To: CCP4BB@JISCMAIL.AC.UK<mailto:CCP4BB@JISCMAIL.AC.UK> Subject: [ccp4bb] MR for coiled coil structure Dear all, We recently acquired a data set (2.0 A, P222) for a coiled coil protein (according to Itasser

Re: [ccp4bb] MR for coiled coil structure

Dear Mark and Shengyang, indeed, as Mark pointed out, we have a special mode in ARCIMBOLDO_LITE devised to tackle coiled-coil structures. Trough the ccp4i interface this will be available as an option you can tick when running an ARCIMBOLDO_LITE job. A tutorial is available on our website (

Re: [ccp4bb] MR for coiled coil structure

This sounds like a case for the Arcimboldo program developed by the Uson group (http://chango.ibmb.csic.es/ ), of which three versions are in CCP4 (Borges, Lite and Shredder). This will use short secondary structure elements in a mixed MR/ab initio approach. We

Re: [ccp4bb] MR for coiled coil structure

Dear Shengyang We'll be happy to help you sort this out. I suggest we discuss it between the two of us and then post a summary to the bulletin board. Could you please tell me, privately not to the board, which gui you are using, which version of CCP4 and which options you are trying to

[ccp4bb] MR for coiled coil structure

Dear all, We recently acquired a data set (2.0 A, P222) for a coiled coil protein (according to Itasser, QUARK, Robetta, and Phaser). Matthews coefficient indicates 1 copy of protein per ASU. Sequence of the protein is quite novel with no apparent homolog in PDB. We tried to to MR with various