Hi,
In addition to all the great suggestions, you can also look into using
Rosetta_MR/Phenix and CNS DEN refinement. Can be useful if all fine with your
data and space group analysis and primary issue is remote model
homology/significant model dissimilarity.
Rosetta MR:
http://www.nature.com/na
I would try harder to get an MR solution from your best homologue.
Things I would do..
1] Check data quality - CCP4I2 gives you a good report - Are there ice
rings? strange wilson plot? missing low resolution data? etc etc..
2] is there Non xrystallographic translation? ccould you have the spece
Dear Madhu,
At the resolution that you mention, which is at the edge of the resolution
limit for ARCIMBOLDO_LITE, and considering that you have already some
information about the possible fold, I would suggest you to use our tool
ARCIMBOLDO_SHREDDER, as it will derive fragments starting from your
Dear all,
We are trying to solve a protein structure of 37KDa using molecular
replacement method. Protein secondary structure indicates that it has
4-Heat repeat (α-helical hair pin) i.e. 16 α-helices. It has about 40%
sequence identity with templates in PDB, using which we tried MR, but we
are