Ronaldo
I have a database of crystallization conditions extracted from the
PDB. I was able to parse the data to extract the concentration of
protein in around 900 cases.
The lowest 9 protein concentrations were as follows:
PDB ID TYPEDATEProtein conc, mg/ml
1EYMISOMERASE.
Just to add that in one case we were able to get suitable crystals after using a
protein concentration of as low as 1.5 mg/ml (kapetaniou et al. Acta Cryst.
2005, F61, 479-481) and microdialysis to slowly remove the excess of salt.
Lower concentrations (0.50-0.75 mg/ml) could also produce crystals
Dear all,
As protein crystallization is the main topic of the day may I include
another question?
What was the minimun protein concentration reported with success in
crystallization trials?
I ask that because the protein I am trying to crystallize is much less
soluble than the one mentioned in
