Dear all,
I would like to thank everyone for being so kind and sparing their time to
respond. Just to summarise from all the responses that I got that may help
someone else:
MBP on its own is monomeric and tends to 'magically' help pretty much every
protein you throw at it to become soluble. In
Dear all,
I would like to ask if someone has experience with maltose binding protein
(MBP) as a tag.
My protein fused to MBP seems to form oligomers that I find difficult to
prevent and I was wondering if mbp behaves similar to gst and may also
be prone to dimerisation/oligomerisation
Many thanks
: Wednesday, July 24, 2013 3:07 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] Maltose binding protein as a tag
Dear all,
I would like to ask if someone has experience with maltose binding protein
(MBP) as a tag.
My protein fused to MBP seems to form oligomers that I find difficult to
prevent
: [ccp4bb] Maltose binding protein as a tag
Dear Kostas,
MBP by itself is a monomer. In most of our cases using it as a fusion tag, the
yield of a target protein increases drastically (i.e. 5 to 20 folds more).
However, we have seen in a couple of cases that the target proteins strongly
interact
