Re: [ccp4bb] Molecular Replacement at low resolution

2008-04-18 Thread Preben Morth
Hi Junyu It is possible at 6 Å resolution, what I would try is to cut your data and try searching in different resolution ranges 20-7 or 25-6.5 , if you are uncertain about your search model, try with a higher RMS value try going up to 2.0. If you have any data, even at very low resolution,

[ccp4bb] Molecular Replacement at low resolution

2008-04-17 Thread Junyu Xiao
Hi, Does anyone have experience with molecular replacement at low resolution? I have a 6Å dataset with probably 3-4 monomers per ASU. Phaser doesn't seem to give me clear results. Can I get some advice? Any comment will be appreciated. Thanks a lot, Junyu