CCP4 bulletin board wrote on 05/22/2008 10:47:24
AM:
> Dear All
>
> I am processing data from a crystal for a large macromolecular complex
> with mosflm. Cell dimensions are around 120 x 150 x 650, with a p222
> spacegroup. To avoid overlaps, we have collected data with a oscillation
> of 0.1 de
Hi Francisco,
you could try using these keywords in Mosflm:
POSTREF WIDTH 3
#since you have 0.1 degree oscillation and Mosflm can only handle 30
images at once, the default I believe is 5 images if I'm not mistaken
SEPARATION CLOSE
PROFILE RMSBG 25
POSTREF USEBEAM
Then I would start indexing
Dear Francisco,
With a c axis of 650 Angs your phi-overlap problem is severe. When this
axis is close to being parallel to the beam, the angular distance in radians
between h,k,l and h,k,l+1 for a reflection hkl close to the top or bottom of
an image is the angle spanned by c* viewed at a dis
AIL PROTECTED]>
To:
Sent: Thursday, May 22, 2008 9:47 AM
Subject: [ccp4bb] Mosflm : data process of crystal with huge unit cell
Dear All
I am processing data from a crystal for a large macromolecular complex
with mosflm. Cell dimensions are around 120 x 150 x 650, with a p222
spacegroup. To
Dear Francisco,
It seems that you are encountering the so-called "phi-overlap" problem,
which would be quie acute when your very long c axis gets close to being
parallel to the beam. In this case, even if you are doing fine slicing as
you are doing here with 0.1 degree images, the mosaicity o
Franciso,
Mosflm does not do a good job refining mosaicity for narrow oscillation
ranges such as you used. I would set the mosaicity to a value that
predicts a number of spots that matches what you actually see and keep
it fixed. Depending on how large your mosaicity actually is, mosflm may
a
CTED]
Sent: Thu 22/05/2008 3:47 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] Mosflm : data process of crystal with huge unit cell
Dear All
I am processing data from a crystal for a large macromolecular complex
with mosflm. Cell dimensions are around 120 x 150 x 650, with a p222
spacegroup.
Dear All
I am processing data from a crystal for a large macromolecular complex
with mosflm. Cell dimensions are around 120 x 150 x 650, with a p222
spacegroup. To avoid overlaps, we have collected data with a oscillation
of 0.1 degrees.
When I try to process the data with mosflm, mosaicity decr
