Lei,
1. Consider making the complex and then purifying the excess peptide
away by dialysis (size exclusion may be tricky since complex may be
diluted in the process).
2. Conventional wisdom would be to try to minimize the amount of excess
peptide as it may "interfere with crystallization". But c
Hello everyone
first I want to thank you guys in advance. I am trying to co-crystallize a 12
KDa protein with a 2 KDa peptide. The biochemistry studies showed the binding
should be 1:1 . but the binding is not very strong ( Kd is around 20-40 uM). I
am wondering, is there any bad effect of e
