If you have hopes for cysteine residues in reasonable proximity, then
bis-iodoacetamide (with a suitable spacer, commercially available is the
ethylenediamine spacer). Any reasonable chemist can make you other spacer
lengths to order.
Homobifunctional PEG with maleimide, N-hydroxy succinimide
Dear all,
Sorry for the off-topic question. I need to prepare an heterodimeric protein
complex for an immunisation, and I would like to make sure that the dimer will
be stable and it won't dissociate after injection.
I am wondering if any of you has a mild cross-linking protocol (and
