Re: [ccp4bb] Off-topic question about SEC

2017-01-12 Thread Nicolas RICHET
> on behalf of Reza > Khayat <rkha...@ccny.cuny.edu> > Reply-To: Reza Khayat <rkha...@ccny.cuny.edu> > Date: Wednesday, January 11, 2017 6:42 PM > To: "CCP4BB@JISCMAIL.AC.UK" <CCP4BB@JISCMAIL.AC.UK> > Subject: Re: [ccp4bb] Off-topic question about SEC > > ​All

Re: [ccp4bb] Off-topic question about SEC

2017-01-12 Thread Nicolas FOOS
t;> Reply-To: Reza Khayat <rkha...@ccny.cuny.edu <mailto:rkha...@ccny.cuny.edu>> Date: Wednesday, January 11, 2017 6:42 PM To: "CCP4BB@JISCMAIL.AC.UK <mailto:CCP4BB@JISCMAIL.AC.UK>" <CCP4BB@JISCMAIL.AC.UK <mailto:CCP4BB@JISCMAIL.AC.UK>> Subject: Re: [cc

Re: [ccp4bb] Off-topic question about SEC

2017-01-11 Thread Christopher Colbert
t <rkha...@ccny.cuny.edu<mailto:rkha...@ccny.cuny.edu>> Date: Wednesday, January 11, 2017 6:42 PM To: "CCP4BB@JISCMAIL.AC.UK<mailto:CCP4BB@JISCMAIL.AC.UK>" <CCP4BB@JISCMAIL.AC.UK<mailto:CCP4BB@JISCMAIL.AC.UK>> Subject: Re: [ccp4bb] Off-topic question about SEC ​

Re: [ccp4bb] Off-topic question about SEC

2017-01-11 Thread Keller, Jacob
Whoa! Big change! Any possible physiological relevance? JPK From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Reza Khayat Sent: Wednesday, January 11, 2017 7:43 PM To: CCP4BB@JISCMAIL.AC.UK Subject: Re: [ccp4bb] Off-topic question about SEC ​All these make sense. Protein

Re: [ccp4bb] Off-topic question about SEC

2017-01-11 Thread Keller, Jacob
Yes if it either A) oligomerizes B) significantly changes shape C) aggregates reversibly On option B: Lower NaCl could make the protein “appear” bigger by unfolding it a bit; hydrophobic interactions should be weaker in lower NaCl. JPK Artem www.harkerbio.com

Re: [ccp4bb] Off-topic question about SEC

2017-01-11 Thread Artem Evdokimov
Yes if it either A) oligomerizes B) significantly changes shape C) aggregates reversibly Artem www.harkerbio.com "where wild SEC columns roam free" On Jan 11, 2017 7:22 PM, "Reza Khayat" wrote: > Hi, > > > Sorry for the off-topic question. Can a protein in lower [NaC]

[ccp4bb] Off-topic question about SEC

2017-01-11 Thread Reza Khayat
Hi, Sorry for the off-topic question. Can a protein in lower [NaC] run faster on a SEC than at higher [NaCl] (i.e. elute at an earlier volume)? The protein elutes well within the resolution limits of the SEC with a symmetric gaussian A280 profile. I know that at lower [NaCl] the protein can