; Herman
>
> Von: CCP4 bulletin board Im Auftrag von Patrick Loll
> Gesendet: Mittwoch, 15. September 2021 15:12
> An: CCP4BB@JISCMAIL.AC.UK
> Betreff: Re: [ccp4bb] Phosphatase enzymatic assay
>
> Phos-tag gels are an optio
It may also be possible to separate the different phosphorylated forms on an
isoelectric focusing gel.
Best,
Herman
Von: CCP4 bulletin board Im Auftrag von Patrick Loll
Gesendet: Mittwoch, 15. September 2021 15:12
An: CCP4BB@JISCMAIL.AC.UK
Betreff: Re: [ccp4bb] Phosphatase enzymatic assay
Phos
Hi,
Could you radiolabel the kinase? If yes, measuring the labeled Pi may work.
Cheers,
Seema
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Phos-tag gels are an option (albeit a fiddly one).
Cheers,
Pat Loll
___
Patrick J. Loll, PhD (he, him, his)
Professor of Biochemistry & Molecular Biology
Drexel University College of Medicine
Room 10-102 New College Building
245 N. 15th St.
Philadelphia, PA 19102-1192
Hello,
try mass spectrometry, either after tryptic digestion into peptides or
electrospray MS on the entire protein if it is not too big.
Best
Wim
- Mail original -
De: "Andrea Moretti"
À: "CCP4BB"
Envoyé: Mercredi 15 Septembre 2021 14:28:45
Objet: [ccp4bb] Phospha
Dear CCP4 community,
sorry for the off topic question but I am struggling with enzymatic
assays and I would need your help.
I am trying to measure dephosphorylation of a phosphorylated kinase by a
ser/thr phosphatase. So far I tried to measure it by malachite green
assay, anti pSer/pThr
