I did crystallise a protein expressed in M. smegmatis a while ago (early 90's)! The cloning was done by Ying Zhang:https://www.jhsph.edu/faculty/directory/profile/786/ying-zhangIt might be worth dropping him a line. That's all I can suggest, sorry!!Good luck.Jon CooperOn 7 Jul 2020 10:07, Matthew
Hi all
I'm designing genes for expression in M. smegmatis (safe host for Mtb
proteins), but its not possible (or advisable due to the GC content) to
optimise for
mycobacterial expression.
Would anyone with experience be able to tell me if its fine to stick with the
E. coli codon optimisation,
