Dear CCP4
I looped a v.thin rod emerging from a cluster of v.thin rods that grew in
29%PEG1500 and 0.1M SPG buffer at pH7.5 (succinic acid, sodium dihydrogen
orthophospate and glycine). The loop i used had been washed more than 10 times
with deionised water (so assumed as 'clean'). The
Hi,
You're working with a very 'rich' crystallization condition. It probably was
supersaturated or close to super-saturated with respect to something, and
that something crashed out on the surface (where liquid contacted air)
forming a crust. Your loop (while perfectly clean) can also be the
The shell may be denatured protein. Remove the protein from the
experiment and the problem will likely go away.
On 11/25/10 09:45, Rick wrote:
Dear CCP4
I looped a v.thin rod emerging from a cluster of v.thin rods that grew in
29%PEG1500 and 0.1M SPG buffer at pH7.5 (succinic acid, sodium
@JISCMAIL.AC.UK
Copie à :
Objet : [ccp4bb] Tough 'shell' on disturbed drop
Dear CCP4
I looped a v.thin rod emerging from a cluster of v.thin rods that grew in
29%PEG1500 and 0.1M SPG buffer at pH7.5 (succinic acid, sodium dihydrogen
orthophospate and glycine). The loop i used had been
@JISCMAIL.AC.UK
Copie à :
Objet : [ccp4bb] Tough 'shell' on disturbed drop
Dear CCP4
I looped a v.thin rod emerging from a cluster of v.thin rods
that grew in 29%PEG1500 and 0.1M SPG buffer at pH7.5 (succinic
acid, sodium
Often you can also avoid this skin formation by adding a bit of your reservoir
solution first to make it a larger droplet.
Then the microtools as mentioned earlier or just two loops
Jürgen
..
Jürgen Bosch
Johns Hopkins Bloomberg School of Public Health
Department of
