On Mon, 20 Apr 2015 23:15:17 +, Keller, Jacob kell...@janelia.hhmi.org
wrote:
I have processed test data sets (measured at the SLS) which have only a few
non-zero pixels on each frame. Can be nicely integrated - but it was insulin.
What were the values of the non-zero pixels, I wonder?
Burning/frying the crystal is never the right strategy, at least not if you
use a Pilatus detector.
Burning/frying is a strategy that results from the widespread misunderstanding
of using Rsym
as a criterion of data quality.
I apologise if I didn't make myself clear. The point I tried to make
On Fri, 17 Apr 2015 15:00:12 +, Keller, Jacob kell...@janelia.hhmi.org
wrote:
Finally, there is simply no downside in collecting more degrees with
proportionally lower dose on the Pilatus. Merging the data recovers the
_same_ signal. It has only advantages - so many that I won't write
I have processed test data sets (measured at the SLS) which have only a few
non-zero pixels on each frame. Can be nicely integrated - but it was insulin.
What were the values of the non-zero pixels, I wonder? How many total counts
per image? And this was, I assume, Pilatus? Sounds pretty
On Fri, 17 Apr 2015 13:02:01 +, Antonio Ariza antonio.ar...@path.ox.ac.uk
wrote:
Hi,
I agree with Kay, try to fry your native crystals to get the highest overall
resolution possible,
since you mention my name in your sentence: no, this is not what I think (nor
said). Burning/frying the
Hi,
I agree with Kay, try to fry your native crystals to get the highest overall
resolution possible, but go for low resolution if your crystals decay rapidly,
particularly when collecting anomalous data. A high overall resolution is
always desirable, but during anomalous phasing you can
Just to clarify, I think what Kay meant with strategy is that you don't just
shoot at the crystal and collect. You should figure out what is the optimum
start and end point of your data collection. Best to be cautious and not
immediately go for highest resolution and not fry your crystal. A 4 A
Something else you could try is using a kappa goniometer. There were a couple
of presentations at the last CCP4 meeting where they discussed that alignment
along the appropriate axis using a Kappa goniometer often worked much better
than the inverse beam mode. By aligning the crystal with the
I would disagree.
My philosophy is: assume this is your only diffracting crystal, maximize the
outcome by investing some thoughts into it before being sorry. Therefore, run
strategy and optimize for anomalous pairs being collected as close in time as
possible.
If you have the luxury of having
Hi,
I'd say using a Pilatus detector in fine-slicing mode and lowdose/high
multiplicity will give you better chances to solve the structure. The right
strategy makes a difference ...
Best,
Kay
But if you only have a single diffracting crystal, then you don't know the
space group before the experiment, and you have to collect 180 (native) or 360
(anom) anyway.
Plus, I have seen too many sorry cases where people thought they had a certain
space group, and later it turned out to be
Finally, there is simply no downside in collecting more degrees with
proportionally lower dose on the Pilatus. Merging the data recovers the _same_
signal. It has only advantages - so many that I won't write them up here with
1 finger on my tablet.
...Up to the point at which one can no longer
I’m sure James Holton has an option for that :-)
By the way, zero photon data sets exist and have been published before (some of
them had to be retracted though).
Jürgen
..
Jürgen Bosch
Johns Hopkins University
Bloomberg School of Public Health
Department of Biochemistry
Hi,
Re Has anybody ever used xenon? Can anyone share the starting pressure
and time for optimization?
This paper :-
doi:10.1107/S0907444901009350 http://dx.doi.org/10.1107/S0907444901009350
offers you details.
Best wishes,
John
On Fri, Apr 17, 2015 at 2:11 AM, joy yang joybeiy...@gmail.com
Hi All,
Thanks a lot for the advice from Mark.
1. For the comment to use a attenuated beam, the major concern here is
resolution, since at full beam, the resolution is already somewhere
between 3.5-4.2, I assume that significant attenuation will bring down the
resolution dramatically, and also
Hi all,
I am working a extremely difficult crystal whose resolution is low (3.5-4 A
for native ) and could not be solved by MR. I am looking for some advice on
data collection strategies, bellow are the details:
Say that I have a rod shaped xtal which is 50 um * 150 um * 20 uM, and if i
use a
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