Dear Rongjin,
I would:
-prepare different cryosolutions, adding glycerol, replacing water
with glycerol, replacing ethanol with other more cryogenic alcohols:
if you have enough crystals, see how these behave when transferred to
these solutions, if they crack, decrease precipitant, if they
Dear All
I got crystals from 20% Ethanol with 0.1M Tris pH 8.5. This is my first
time to have crystals in Ethanol and want to get some suggestions of
cryo-protection from those who have done this before.
I am waiting for my time on home X-ray facility, and hope I can get
some suggestions before
Hi Rongjin,
I had crystals that grew in 9% ethanol as a precipitant. To cryoprotect
them, I first replaced the mother liquor with solution containing 10% MPD,
instead of ethanol, and then briefly dunked the crystals into ~30% MPD.
Hope this helps. Good luck.
Katya
On Mon, Sep 29, 2008 at 1:24
