[ccp4bb] crystal bent once open cover slip

Hi Folks, We have some membrane protein crystals that are grown in 30%PEG400, 0.1M Na Citrate pH 4.5, 0.1M LiCl. The protein is purified in DDM. The crystals are long rods and grown under room temperature in a hanging drop set up. But once we open the cover slip, we see the rods start to

Re: [ccp4bb] crystal bent once open cover slip

You probably use hanging drops. It's the surface tension effect. Check if sitting drops are better. Maia Sitting drops weikai wrote: Hi Folks, We have some membrane protein crystals that are grown in 30%PEG400, 0.1M Na Citrate pH 4.5, 0.1M LiCl. The protein is purified in DDM. The

Re: [ccp4bb] crystal bent once open cover slip

you could try sitting drops. Perhaps you can fish a crystal quicker, before it degrades. In sitting drops the crystals may also be further away from the drop surface and take longer to degrade. Or quickly add mineral oil to cover the sitting drop and fish the crystals through the oil. If

Re: [ccp4bb] crystal bent once open cover slip

Weikai, What you might be experiencing is a detergent effect, i.e., you are near a detergent-dependent crystallization boundary. We have been hit with this many times. Under vapor diffusion conditions, sitting or hanging drop, the protein-detergent complex crystallizes and the free/