[ccp4bb] deglycosylation of proteins expressed in mammalian cells at room temperature or lower

2009-06-02 Thread Jerry McCully
Dear All: Recent we expressed one protein using mammalian cells but this protein was highly glycosylated (30% of the total molecular weight). We want to remove some carbohydrate by enzymatic digestion for the convenience of crystallization. However, this protein tends to

Re: [ccp4bb] deglycosylation of proteins expressed in mammalian cells at room temperature or lower

2009-06-02 Thread Pius Padayatti
will work fine but leave overnight. psp On Tue, Jun 2, 2009 at 5:36 PM, Jerry McCully for-crystallizai...@hotmail.com wrote: Dear All:    Recent we expressed one protein using mammalian cells but this protein was highly glycosylated (30% of the total molecular weight).    We want to