We chewed pills with EtBr as kids in school to see if we brushed our teeth well
- red colour on the edges, bad boy
Poul
On 01/10/2011, at 19.12, Jacob Keller j-kell...@fsm.northwestern.edu wrote:
I actually looked at an EtBr MSDS a while ago, and was shocked at how
benign it was. I also
Jacob Keller wrote:
I actually looked at an EtBr MSDS a while ago, and was shocked at how
benign it was. I also heard from someone that they used to feed it to
Argentinian cows routinely a few years back...
Wikipedia says it was used as a trypanosomacidal - It's being
discontinued not because
There exists a less toxic chemical than EtBr to stain DNA: SYBR safe
DNA stain (a fluorescence dye sold by a certain vendor). Another
benefit is to be able to use blue light, reducing UV/VIS light
exposure when handling gels.
Florian
On Oct 2, 2011, at 11:49 AM, Edward A. Berry wrote:
There exists a less toxic chemical than EtBr to stain DNA: SYBR safe
DNA stain (a fluorescence dye sold by a certain vendor).
SYBR Safe is about 10X less sensitive though.
I suspect that not many chemicals in the lab are less toxic/mutagenic than
EthBr. The classic Ames test shows that 5 ug
i wouldn't recoommend that. here is the info from somebody forwarded
from our genetics department with regards to safety of that while
back
Sybr is just as toxic/poisonous/harmful as ethidium bromide, only
far more expensive. There have been very few tests concerning it's
use up
There exists a less toxic chemical than EtBr to stain DNA: SYBR safe
DNA stain (a fluorescence dye sold by a certain vendor).
SYBR Safe is about 10X less sensitive though.
Can you do the toothbrush test with SYBR Safe?
I wouldn't do that. As it is considerably more hydrophobic, I'd expect
If you can reproduce the crystals and have the material
1. Harvest several large crystals.
2. Make several transfers to fresh mother liquor to wash.
3. Dissolve in DNA loading dye without SDS
4. Run on a native gel (e.g. 6% polyacrylamide, 0.5XTBE, etc.).
5. Include positive control lanes for
Also this method might be useful if crystals tolerate the treatment.
Regards,
Juha
Fluorescence detection of nucleic acids and proteins in multi-component
crystalshttp://onlinelibrary.wiley.com/doi/10.1107/S0907444905035365/abstractActa
Crystallographica Section D
Volume 62, Issue 2, February
If your DNA is small enough, you can run a control dsDNA lane on the gel. dsDNA
will show up on silver stain but the oligomer has to be small enough to enter
the gel.
Kay
On Sep 30, 2011, at 10:37 PM, zq deng dengzq1...@gmail.com wrote:
Hi all,
.
recently,I got a crystal of protein-DNA
+0800
From: CCP4 bulletin board CCP4BB@JISCMAIL.AC.UK (on behalf of zq deng
dengzq1...@gmail.com)
Subject: [ccp4bb] detect dsDNA
To: CCP4BB@JISCMAIL.AC.UK
Hi all,
.
recently,I got a crystal of protein-DNA crystal.i
used silver stainto prove that it is a protein
crystal.Does anyone
I actually looked at an EtBr MSDS a while ago, and was shocked at how
benign it was. I also heard from someone that they used to feed it to
Argentinian cows routinely a few years back...
JPK
On Sat, Oct 1, 2011 at 1:56 AM, James Stroud xtald...@gmail.com wrote:
If you can reproduce the crystals
Hi all,
.
recently,I got a crystal of protein-DNA crystal.i used silver stainto prove
that it is a protein crystal.Does anyone have method to detect if there is
DNA in the crystal.
any suggestion will be appreciated.
Regards,
deng
board [CCP4BB@JISCMAIL.AC.UK] On Behalf Of zq deng
[dengzq1...@gmail.com]
Sent: Friday, September 30, 2011 8:36 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] detect dsDNA
Hi all,
.
recently,I got a crystal of protein-DNA crystal.i used silver stainto prove
that it is a protein crystal.Does
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