[ccp4bb] different Kd and Km value

Hi I have a protein with two substrate. when I am doing the binding studies with the two substrate separately, I am finding one of the substrate to have similar kd and Km. however the km and kd values are almost 30 fold different for the other substrate. it binds 30 fold more tightly then

Re: [ccp4bb] different Kd and Km value

On Feb 26, 2015, at 4:51 PM, Srivastava, Dhiraj dhiraj-srivast...@uiowa.edu wrote: Hi I have a protein with two substrate. when I am doing the binding studies with the two substrate separately, I am finding one of the substrate to have similar kd and Km. however the km and kd values