Chris
Isopropanol is said a very good precipitant but it's unpopular because it's
so difficult to harvest crystals
However there's a very convenient way to grow and harvest crystals in
isopropanol using Vapor Batch plates.
Basically you set up microbatch-under-oil drops containing all the
Dear all,
I have a protein which crystallizes in 25% isopropanol, at pH4.5.
Does anyone have experience freezing crystals grown in such a condition?
What cryoprotectants should I try?
Can isopropanol itself act as a cryoprotectant?
Any suggestions on how to deal with isopropanol evaporation
From: CCP4 bulletin board [mailto:ccp...@jiscmail.ac.uk] On
Behalf Of Chris Meier
Sent: Friday, April 09, 2010 10:39 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] freezing crystals grown in isopropanol
condition
Yes, isopropanol is a cryoprotectant, and a relatively good one. So are
the other alcohols. It was even popular in the olden days when we
would typically set up drops that were 5-10 microliters in volume
(each!). These take a while (minutes) to evaporate, giving you enough
working time to
I have wondered if placing a layer of oil over the drop would help
solve the problem of the crystals moving around. Haven't tried it,
but don't people harvest from a microbatch tray by dragging the loop
and crystal through oil?
Nat
On Fri, Apr 9, 2010 at 11:21 AM, James Holton jmhol...@lbl.gov
Yes, oil is great, but you have to be careful to choose an oil in which
the alcohol is not soluble, or the oil will suck it out of your drop,
(just like air). This is particularly annoying with detergents, which
are almost all soluble in oil. I've always thought that maybe some
synthetic
Nat,
A few years ago I had K channel crystals that formed under similar
conditions. I found that using MPD as a cryo-protectant worked best. As
for the evaporation issue, I had a little extra time as I was performing the
mounting in a cold room.
Scott
Pegan S, Arrabit C, Zhou W, Kwiatkowski
