Does one regard the metal atom in a metalloprotein as being part of the protein?
If so, a shared metal could occupy a special position in a dimer for example.
In Acta Cryst. (2008). D64, 257-263 Metals in proteins: correlation between
the metal-ion type, coordination number and the amino-acid
Colin Nave wrote:
..
Also Acta Cryst. (2002). D58, 29-38 The 2.6 Å resolution structure of Rhodobacter
capsulatus bacterioferritin with metal-free dinuclear site and heme iron in a
crystallographic `special position' D. Cobessi, L.-S. Huang, M. Ban, N. G. Pon, F.
Daldal and E. A. Berry (
Good point Colin! 2-Zn insulin is of course a classic example of
this, where the two independent Zn2+ ions both sit on the
crystallographic 3-fold in R3. It doesn't matter whether you count
the metal ion as part of the protein or not: if I understand Gloria's
original question correctly, all
The proper occupancy for an atom on a special position depends on
how one defines the meaning of the number in that column. In the
past, refinement programs, at least I know mine did, simply expanded
all atoms in the coordinate file by the symmetry operators to determine
the contents of the
No that surely can't be right. Application of a symmetry operator to
a point on a special position which is unchanged by the operator
doesn't generate a symmetry copy of the point, because there is no
symmetry copy of such a point! For general Wyckoff positions it does,
for sure. If you look at
On Fri, 2010-12-10 at 22:40 +, Ian Tickle wrote:
Application of a symmetry operator to
a point on a special position which is unchanged by the operator
doesn't generate a symmetry copy of the point, because there is no
symmetry copy of such a point!
Why not? Symmetry-related copy may be
: Saturday, December 11, 2010 0:41
Subject: Re: [ccp4bb] Fwd: [ccp4bb] Wyckoff positions and protein atoms
To: CCP4BB@JISCMAIL.AC.UK
No that surely can't be right. Application of a symmetry
operator to
a point on a special position which is unchanged by the operator
doesn't generate a symmetry
SHELXL also expects that the occupancy of a fully occupied atom on a
threefold axis should be set at 1/3, and will generate this automatically
if necessary. It will also generate automatically the necessary
constraints for the x, y and z parameters (and for the Uij if the atom is
anisotropic).
No disorder is involved.
The occupancy of an (fully occupied) atom on an n-fold rotation axis is
1/n
If a two-fold, 1/2
If a three-fold, 1/3
When you sum over all the atoms in the unit cell, application of the
symmetry operations to atoms lying on the rotation axis generates atoms
] On Behalf Of
Ralf W. Grosse-Kunstleve
Sent: Thursday, December 09, 2010 3:47 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] Fwd: [ccp4bb] Wyckoff positions and protein atoms
Hi Gloria,
My hobby is space group symmetry.
My interest phenix development.
so I can't imagine a protein crystallographer
[mailto:ccp...@jiscmail.ac.uk] On Behalf Of
Ralf W. Grosse-Kunstleve
Sent: Thursday, December 09, 2010 3:47 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] Fwd: [ccp4bb] Wyckoff positions and protein atoms
Hi Gloria,
My hobby is space group symmetry.
My interest phenix development.
so I can't
cases out there (and so far I have heard of a disulfide bond on a
2-fold connecting two homodimers).
I'm slightly puzzled by this example. If the S-S bond is on the
special position, then the rest of the molecule can't have 2-fold
symmetry, so would have to be rotationally disordered with
will occupy true Wyckoff
positions.
Best,
Herman
-Original Message-
From: CCP4 bulletin board [mailto:ccp...@jiscmail.ac.uk] On Behalf Of
Ian Tickle
Sent: Thursday, December 09, 2010 3:35 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] Fwd: [ccp4bb] Wyckoff positions and protein atoms
-Original Message-
From: CCP4 bulletin board [mailto:ccp...@jiscmail.ac.uk] On Behalf Of
Ian Tickle
Sent: Thursday, December 09, 2010 3:35 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] Fwd: [ccp4bb] Wyckoff positions and protein atoms
cases out there (and so far I have heard of a disulfide
I've gotten some interesting responses, that I will summarize for the
group later, but I thought I should clarify why I asked.
I was worrying about this because I have been working out the steps in
how to determine the (3+1)D superspace group for a protein crystal.
The last step listed in IT vol
to handle them in a special way. So Wyckoff
positions remain foreign in the macromolecular context.
Ralf
- Original Message
From: Gloria Borgstahl gborgst...@gmail.com
To: CCP4BB@JISCMAIL.AC.UK
Sent: Wed, December 8, 2010 12:16:54 PM
Subject: [ccp4bb] Fwd: [ccp4bb] Wyckoff positions
Original Message
Subject:Re: [ccp4bb] Space group and R/Rfree value
Date: Wed, 01 Dec 2010 09:44:06 -0700
From: Maia Cherney ch...@ualberta.ca
To: Xiaopeng Hu huxp...@mail.sysu.edu.cn
References:
643947201.129232.1291191478190.javamail.r...@zmbx0.sysu.edu.cn
---BeginMessage---
Kay Diederichs wrote:
Eric Karg harvard...@yahoo.com
Datum:
Sun, 14 Nov 2010 21:37:10 +
Dear all,
Thanks for your suggestions. From what I learned new GPUs from NVIDIA are using
the Optimus technology which does not support Linux, meaning that only the
dedicated
On Thu, Oct 21, 2010 at 11:42 AM, Ian Tickle ianj...@gmail.com wrote:
There are a number of things that don't look right here, both with the
Refmac and the Phenix runs: [...]
interesting, thanks for these comments.
S should not have been symmetrized
to actually display the principal axes in
... maybe, to clarifiy my question a little bit: I want to fill an
essentially flat cryo-EM-map with dummy atoms. So, a peak search doesn't
work. I would need a program that fills this map with dummy atoms for a
few things that I want to try with this atomic representation.
Best regards,
Doesnt arp/warp start with doing something like this? If you gave a 0/1
mask I wonder what the first build would look like..
You would have to invent a reflection file for the map ...
E
On 10/13/2010 12:49 PM, Dirk Kostrewa wrote:
... maybe, to clarifiy my question a little bit: I want to
Hi Folks,
The paper Phil recommended looks very nice. Below is a summary when
last time I asked people about this.
Regards,
Weikai
Original Message
Subject: [ccp4bb] Summary: measure detergent concentration
Date: Wed, 28 Oct 2009 16:49:32 -0400
From:
There is just over a week left to apply for this X-Ray Lab Manager job in
central London.
Best wishes
Nick
--
Prof Nicholas H. Keep
Executive Dean of School of Science
Professor of Biomolecular Science
Crystallography, Institute for Structural and Molecular Biology,
Department of Biological
Apologies if this has been posted already but I couldn't see it yet!
Hi,
Sorry for the off topic question!
I'm looking at Dali search results and find the molecule names are not
always the same as the ones for the chain identifier from the pdb
entry (maybe the molecule names are always of
WRT the redundancy, I am afraid you have to recompute an approximate value
yourself using the number of observations and number of unique reflections
(this is what I do all the time). I suppose one could always write a jiffy
program to compute the correct values using both files
Apologies for the repetition, but only the first link:
http://cc.oulu.fi/~pkursula/xdsi.html
is for the xdspub program that produces the output I attached. The other one
has the same name, but does something else!
Luca
Another very nice script to run and collect statistics from XDS is
xdsme from Pierre Legrand
http://code.google.com/p/xdsme/
Daniel
Le 05/08/2010 16:55, Jovine Luca a écrit :
WRT the redundancy, I am afraid you have to recompute an approximate value
yourself using the number of
Prof. Gebhard Schertler
Head of Biology and Chemistry
Biomolecular Research Laboratory
Paul Scherrer Institute
Anfang der weitergeleiteten E-Mail:
Von: Daniel Oprian opr...@brandeis.edu
Datum: 8. Juli 2010 17:16:43 MESZ
An: gebhard schertler gebhard.schert...@psi.ch
Betreff: postdocs
Forgot to also post this response to the BB.
Randy
Begin forwarded message:
From: Randy Read rj...@cam.ac.uk
Date: 25 June 2010 12:59:12 GMT+01:00
To: Yong Y Wang wang_yon...@lilly.com
Subject: Re: [ccp4bb] error in running Phaser NMA mode
Dear Yong,
Sorry about that! I suspect the
Received from Peter Zwart:
Original Message
Subject:Re: [ccp4bb] creating new ligand cif file
Date: Mon, 21 Jun 2010 07:08:18 -0700
From: Peter Zwart phzw...@lbl.gov
To: Vellieux Frederic frederic.velli...@ibs.fr
References: 4c1f4f26.2070...@ibs.fr
it is
This very rapid reply to my withdrawal as a reviewer for NPG journals contains
a response issued last night purporting to clarify issues obfuscated by the UC
Digital Library in their letter. They claim that the data cited by the UCDL are
misleading. I can neither verify or refute this claim.
Well - try the coordinate utility recommended by Martyn Winn to convert
cif to pdb
coord_format xyzin ./1ivo.cif xyzout 1ivo.pdb eof
END
eof
Then
pdbset xyzin 1ivo.pdb xyzout 1ivo-sym.pdb
symgen -x,y,z (or whatever sym op you want)
end
This will generate symmetry copy of your coordinates as
On Tue, May 25, 2010 at 6:58 AM, Pavel Afonine pafon...@lbl.gov wrote:
Yes, because all the information you need is encapsulated in 1 number
per region of interest!
I agree it's a good reason.
Actually what I described doesn't quite achieve this, because you need
to keep a record of both the
On Tue, May 25, 2010 at 6:22 AM, Frank von Delft
frank.vonde...@sgc.ox.ac.uk wrote:
Hi Ian, I read with great interest. But got stumped here:
- how you compute sigma(rho)?
See my reply to George Sheldrick's post.
I think your reply did not make it out to the BB, certainly neither to my
Hi Pavel
Phew! Lots of questions - this could take a while:
- where this formula come from and what are the grounds for this?
It's just the RMSD of the density divided by its standard uncertainty
(sigma), which we're assuming is the same for all grid points: this
isn't quite true, sigma is
Hi Ian,
thanks for very detailed reply!
- do you think it is better than looking at three values {map CC, 2mFo-DFc,
mFo-DFc} and why?
Yes, because all the information you need is encapsulated in 1 number
per region of interest!
I agree it's a good reason.
But I don't understand
On Sun, May 23, 2010 at 5:24 AM, Pavel Afonine pafon...@lbl.gov wrote:
Hi,
On 5/22/10 8:23 PM, Zhang, Hailiang wrote:
Thanks a lot! Actually my map has very weak density at only certain
region, and I want to numerically say that this region is weakly
correlated to the atomic map at this
Original Message
Subject: Is it possible for the Tris buffer to strip the Zn ions from
the Zinc Finger motif of a protein?
Date: Sun, 23 May 2010 08:45:55 -0600
From: Maia Cherney ch...@ualberta.ca
To: ruheng rh_ibp2...@hotmail.com
The complex can dissociate
sometimes change in ph also dissocaites the complex,so you need to screen
best buffer ph for your protein complex.
atul kumar
On Sun, May 23, 2010 at 8:18 PM, Maia Cherney ch...@ualberta.ca wrote:
Original Message
Subject:Is it possible for the Tris buffer to strip
Dear Nasos Hailiang,
Gerard Kleywegt's MAPMAN calculates the
RSR, discussed here:
http://xray.bmc.uu.se/usf/mapman_man.html#S41
Mark
On 21 May 2010 23:54, Athanasios Dousis ndou...@rice.edu wrote:
Hello all,
I'm forwarding a question from my labmate
Hi,
On 5/22/10 8:23 PM, Zhang, Hailiang wrote:
Thanks a lot! Actually my map has very weak density at only certain region, and I want to numerically say that this region is weakly correlated to the atomic map at this region. But according to the CC formula, if investigating by residue, CC only
Hello all,
I'm forwarding a question from my labmate Hailiang Zhang regarding
OVERLAPMAP and real-space R factors.
Thanks in advance for your suggestions.
Nasos Dousis
Rice University
P.S. Can someone please add haili...@bcm.tmc.edu to the CCP4BB listserv?
Original Message
Hello,
I was just wondering whether there is some other tools in eg CCP4, Phenix or
CNS to calculate real-space R in the general way in absolute values.
related to your question: in PHENIX you can get a table of map CC
(computed between model and 2mFo-DFc maps), and the value of mFo-DFc
-- Forwarded message --
From: Arpit Mishra ar...@igib.in
Date: Wed, May 12, 2010 at 6:20 PM
Subject: regarding purification of DNA binding protein
To: ccp...@jiscmail.ac
hi all
i am trying to purify DNA binding protein, but i couldnt get rid of the DNA
after performing hi-trap
Hello Arpit,
don't you use DNAse during lysation of the cells? Or do you mean that you want
to get rid of unspecific DNA and want to add some specific DNA after
purification? In that case I'd understand you don't want to use DNAse.
Tim
On Wed, May 12, 2010 at 06:23:56PM +0530, Arpit Mishra
Hi Arpit,
You can use PEI (polyethyleneimine). Adding PEI at low conc. (e.g. 0.25%)
after cell lysis should precipitate most of the DNA. Note, it is best to do
the addition step-wise at 4 deg with gentle steering over a period of time
10-15 min or so.
Ibrahim
On 5/12/10 10:20 AM, Tim
I mistakenly sent this off to Enrico, rather than to the CCP4BB. Apologies to
Enrico.
Begin forwarded message:
From: Charles W. Carter, Jr car...@med.unc.edu
Date: May 6, 2010 6:50:23 AM EDT
To: est...@cea.fr
Subject: Re: [ccp4bb] control of nucleation
In fact, there is quite good
The Laboratory of Molecular Biology (LMB) in Cambridge, UK wishes to
recruit an Investigator Scientist to support a range of collaborative
projects involving biophysical methods for studying macromolecules and
their interactions. The individual will also conduct workshops and
informal
I have personally noticed that the blue color only appears when the pH of the
hyperquenched solution is higher than 7 or so. I assume this is because
solvated electrons react with protons to form their conjugate base: the
hydrogen atom. The latter species is highly reactive as well, but
@JISCMAIL.AC.UK
Subject: [ccp4bb] Fwd: [ccp4bb] Blue color upon X-ray exposure?
I have personally noticed that the blue color only appears when the pH of the
hyperquenched solution is higher than 7 or so. I assume this is because
solvated electrons react with protons to form their conjugate base
Dear All,
I am currently attempting to phase a protein domain target after soaking the
crystal with Tobias Beck's I3C magic crystal compound, using XDS and SHELX.
After XDS processing, I was able to detect 27% anomalous signal. I used ccp4
to generate an .mtz file. After attempting to convert the
-- Mensagem encaminhada --
De: Educação Física edfisicaufp...@gmail.com
Data: 20 de fevereiro de 2010 19:11
Assunto: Kit Do Brasileiro
Para: Adriene Melo dry-...@hotmail.com, Alysson Simões
alyssonsimo...@hotmail.com, Arthur Araújo arthur_sohs...@hotmail.com,
ass...@hotmail.com,
Begin forwarded message:
From: Charles W. Carter, Jr car...@med.unc.edu
Date: February 19, 2010 10:28:55 AM EST
To: George M. Sheldrick gshe...@shelx.uni-ac.gwdg.de
Subject: Re: [ccp4bb] anomalous difference fourier maps
I'm also inclined to join this discussion. I agree with both Gérard
Hello,
May the pouch of your Holiday Wombat overflow with presents!
I would like to draw your attention to a summer internship in our lab at
Monsanto. If you are an interested student, or know someone who may be
interested - please apply using
http://www.monsanto.com/careers/opportunities/ and
Postdoc Position at UCSD:
A full-time postdoctoral position is available at UC San Diego. We
are looking for a highly motivated postdoctoral research fellow to
join our laboratory. We are interested in structure-function studies
of Protein-DNA and Protein–RNA complexes using a combination
Forgot to hit the Reply all button (sorry :-( )
---BeginMessage---
Katja Schleider wrote:
Hi everybody,
sorry for my off-topic question. I got small initial crystals in 200mM
NaSulfat and 20% PEG 3350.
There is no buffer in this condition. How can I optimize these
crystals? Just vary the PEG
Just thought this will be of interest to all.
Subbu
---BeginMessage---
Narayanan Ramasubbu wrote:
Vellieux Frederic wrote:
Narayanan Ramasubbu wrote:
Hi:
Could someone point out the name and where to get these
crystallization plates used in the video?
By the way, this is a wonderful video.
Oops.., I meant to ask a different quesition. Let me try again:
I used PyMol to generate a qualitative Vacum Electrostatic surface (without
APBS calculations). The potential is displayed in the range -56.3 to +56.3.
Is it possible to change this scale in PyMol, if so how?
Thank you,
-Partha
To measure detergent concentrations in protein samples, the way that I
recommend is ATR-FTIR, it is very accurate, fast (10min ) and
requires as low as 10uL of protein sample.
Here is the reference:
· PVeesler, D. et al. Production and biophysical characterization
of the CorA
Hi there,
Would there be a kind soul that could pass this message on to cnsbb? I
am registered with e-mail address velli...@ibs.fr but the e-mail address
has changed to frederic.velli...@ibs.fr 12 months ago perhaps. I am not
very good at all these e-mail diffusion lists and cannot see how to
I pass the following comment received privately, could be of use to
either the Coot developpers (Paul Kevin) or to simple Coot users (like
me).
Fred.
---BeginMessage---
Fred, the same thing happened to me the other day. All of a sudden, Coot
simply would not do rsr complaining of lacking
---BeginMessage---
NCS. I don't think REFMAC can handle yet NCS (help me there Garib). CNS
FYI there's documentation for NCS options in REFMAC, although I haven't
actually used them yet (haven't been lucky enough to have NCS in my
structures).
Pete
---End Message---
attachment:
This could be due to biscuit contamination of your protein - did you eat cake before setting up your crystallisation drops?Alternatively it could be a Fourier ripple - a very strong 5.4 Å resolution 00l reflection which is not correctPoulBegin forwarded message:From: hayato Jumonji
Subject: IPTG
In response to your email thread discussion please also add
Carbosynth (www.carbosynth.com) offering IPTG 10g $85.They are bulk
producers (100kg batches)
Thanks to all who helped with this, especially Paul Emsley, Ed
Pozharski, Robert Orru, Partha Chakrabarti, and Michael Strickler. Here
is a brief summary of the results:
1. It's easy to compile Coot for 64-bit Ubuntu 9.04 using the autobuild
script *IF* you have all the the prerequisites.
Begin forwarded message:
From: Richard Gillilan r...@cornell.edu
Date: June 23, 2009 9:43:20 AM EDT
To: Nadir T. Mrabet nadir.mra...@medecine.uhp-nancy.fr
Subject: Re: [ccp4bb] structure - function
A very interesting question.
Stephan Jay Gould was well known for his argument that evolution is
Dear crystallographers!
My basic question is, how can electron density maps be moved along with
the
respective PDB coordinates?
I would like to superimpose two structures from different space groups
(P21
and P212121) along with some
omit ligand density in the active center to later highlight the
Sascha M. Marek wrote:
Dear crystallographers!
My basic question is, how can electron density maps be moved along with
the
respective PDB coordinates?
If you have Coot, you can try Extensions - Maps - Transform map by LSQ
model fit.
I hope this helped our Australian friends get more funding!
Ho
The TV show 'Thank God You're Here' goes to the synchrotron!
http://www.youtube.com/watch?v=N_zbySqumaAfeature=related
?
Jacob
- Original Message -
From: Patrick Loll
To: CCP4BB@JISCMAIL.AC.UK
Sent: Friday, March 27, 2009 8:35 AM
Subject: [ccp4bb] Fwd: [ccp4bb] Crystal vacuum cleaner
Pretty cool, but the examples shown are all gigantic. Having just spent a
frustrating several hours chasing
Since Sacha is having trouble posting directly the list I will
forward his latest message since It addresses my second set of
questions:
Begin forwarded message:
From: Alexandre OURJOUMTSEV sa...@igbmc.fr
Thank you, Richard, for your questions !
Unfortunately, I failed to pass my mail to
Hi Uli,
When we send our dry shipper from UK to ESRF, Grenoble, via FedEx we
place a following note on the box:
DRY SHIPPER, Non-hazardous, non-toxic, non-flammable, non-
restricted. Conforms with the IATA regulations Special Provision A800
We write the same in the Contents place of the
wishes
Misha Isupov
University of Exeter
From: CCP4 bulletin board [ccp...@jiscmail.ac.uk] On Behalf Of Stephen Hare
[s.h...@mail.cryst.bbk.ac.uk]
Sent: 06 January 2009 16:35
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] Fwd: Twinning
Dear All,
We
Hey Stephen,
how about simply putting three models, separated by 16A, into your
original unit cell and refining them together. You'd have to guess
their fractional occupancies from the heights of the Patterson peaks
(and make them add up to one, obviously).
This approach (in contrast to
Dear All,
We are currently working on a structure of apparent P21 symmetry which
has been solved by molecular replacement. The data are to 2.7Å but the
Rfree will not drop below 30%. The density is clear for the model we
have, however there is extra density that suggests a shift of the
On Tuesday 06 January 2009 08:35:19 Stephen Hare wrote:
Dear All,
We are currently working on a structure of apparent P21 symmetry which
has been solved by molecular replacement. The data are to 2.7Å but the
Rfree will not drop below 30%. The density is clear for the model we
have,
This is an interesting case but your description lacked the diffraction part.
Assuming no Heavy Atom sites in the native structure. Based on your
description, another possibility may be Lattice Translocation.
-
Ref: J. Wang, S. Kamtekar, A. J. Berman and T. A. Steitz.
Hi there
Assuming you have a model of the complex you are interested in
tinkering with, try submitting it to the Rosetta Alanine Scanning
servery thing.
http://robetta.bakerlab.org/alascansubmit.jsp
By default, it mutates each residue in the interface to ala, does some
local minimisation (side
Satellite Workshop
BioOrbit: coupling methods for exploring structures of increasing
complexity from molecules to tissues
on Monday 19th (afternoon) Tuesday 20th January, 2009
Auditorium Bloch
at CEA L'Orme des Merisiers (next to SOLEIL), Saint Aubin (France)
And this is what I call just in time publication:
Davis et al. RosettaLigand Docking with Full Ligand and Receptor
Flexibility. J Mol Biol (2008) pp.
available online today.
Jürgen
On 3 Dec 2008, at 01:47, David Briggs wrote:
Hi there
Assuming you have a model of the complex you are
Charlie,
This is not a good comparison because combining NMR and X-ray data is
like having higher resolution again (i.e. more constraints).
Further, the informations are much more of a redundant nature than
profoundly complementary as they stem from the same molecule.
More resolution would be
I know it's not CCP4 but I use moleman2 for this, http://xray.bmc.uu.se/usf/xutil.html
. You read your pdb into moleman and choose the STatistics option, it
outputs Rg among lots of other things. It can all be scripted too if
you wanted to say charge through the whole PDB and get Rg for
I recently had exactly this problem only I caught the crystal frozen
in the act of being catapulted out of the loop. I was using a thicker-
than usual oil for cryoprotectant and kept seeing empty loops with
what looked like long clear hairs attached. Finally, one loop had a
graceful arc of
Date: Mon, 14 Jul 2008 15:22:52 -0700 (PDT)
From: Martin.Laurberg [EMAIL PROTECTED]
To: [EMAIL PROTECTED]
Cc: CCP4BB@JISCMAIL.AC.UK
Subject: DNA/RNA base stacking restraint add-on to CNS avaliable.
Dear all,
A DNA/RNA base stacking restraint add-on to CNS version 1.21 is
avaliable
for
I now have Hardy Heron on my EM64T machine, and ccp4 works fine.
For bltwish, I'm still using the version I compiled from source ages
ago, as described on my page. Did you try that?
Martyn
Forwarded Message
From: Tim Gruene [EMAIL PROTECTED]
Reply-To: Tim Gruene [EMAIL
Tommi,
The question has been asked and answered not by protein
crystallography, but by cyroelectron microscopy and EM freeze etch
research. Even as far back as the early 1960's, people noticed that
liq. N2 was really slow at cooling. Read the cyroEM work on the
bacteriorhodopsin
Dear Vaheh,
I'm not aware of a commercial or cell bank source, but you can get these cells
from HG Khorana's lab at MIT.
Best wishes,
radu
--
A. Radu Aricescu, PhD
University of Oxford
Wellcome Trust Centre for Human Genetics
Division of Structural
Hi Charlie,
yes you are right, but I assumed if people see a cloud of condensed
fog over their LN2 bath they should remove that by
a) filling up the bowl completely e.g. some LN2 drips out of the bowl
b) blow the fog away before you dip
True; this has been demonstrated quite rigorously:
yes you are right, but I assumed if people see a cloud of condensed
fog over their LN2 bath they should remove that by
a) filling up the bowl completely e.g. some LN2 drips out of the bowl
b) blow the fog away before you dip
I think the original poster meant the relatively low heat conduction
I think the important thing here is that liquid nitrogen in the lab
tends to be exactly at its boiling point, since the temperature is
maintained by continuously boiling off some of the N2.
This means the only mechanism for heat absorption is through vaporization,
depending on the latent heat of
according to literature,see below and references
http://www.px.nsls.bnl.gov/courses/papers/ZD_EG_papers.html,
it is not clear that liq. propane plunged item would cool
faster. (whilst i havent tested this)...
Would anyone have actual experimental data with protein crystals
on the
-- Forwarded message --
From: Jayashankar [EMAIL PROTECTED]
Date: Tue, May 20, 2008 at 7:34 PM
Subject: Right terminal residues for constructs.
To: CCP4BB@jiscmail.ac.uk
Dear friends and scientists,
(A pre-Structural biological question.)
I Have a multidomain protein , I know
Dear Joe --
Does anyone have information about how long it takes to set up a 96-
well tray for the crystallization robots available? Besides cost
per tray and maintenance cost, another important feature we
consider is the time for setting up a 96-well tray. It is an
important factor
: [ccp4bb] Fwd: [ccp4bb] crystallisation robot
Hi,
Does anyone have information about how long it takes to set up a
96-well tray for the crystallization robots available? Besides cost
per tray and maintenance cost, another important feature we consider
is the time for setting up a 96-well tray
Thanks Leo for your introduction.
However, the link is broken.
Please look at the following page.
http://pfweis.kek.jp/protein/Robot/PXS/index-e.html
Best regards,
Yusuke
---
Yusuke Yamada, Ph.D.
Photon Factory
High Energy Accelerator Research
**
** Roosevelt Drive [EMAIL PROTECTED] **
** Headington, Oxford OX3 7BNhttp://www.oppf.ox.ac.uk **
Original message
Date: Mon, 14 Apr 2008 17:10:26 -0400
From: JOE CRYSTAL [EMAIL PROTECTED]
Subject: Re: [ccp4bb] Fwd: [ccp4bb] crystallisation
To: mailto:CCP4BB@JISCMAIL.AC.UKCCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] Fwd: [ccp4bb] crystallisation robot
One thing that people often overlook is that quite a lot of protein
can be lost by denaturation on the surface of the drop. This is more
significant for smaller drops. Two suggestions: (1) increase
Subject: [ccp4bb] Fwd: [ccp4bb] crystallisation robot
One thing that people often overlook is that quite a lot of protein
can be lost by denaturation on the surface of the drop. This is more
significant for smaller drops. Two suggestions: (1) increase the
proportion of protein
PROTECTED] On Behalf Of
Patrick Shaw Stewart
Sent: Friday, January 18, 2008 2:20 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] Fwd: [ccp4bb] crystallisation robot
One thing that people often overlook is that quite a lot of protein
can be lost by denaturation on the surface of the drop. This is more
board [mailto:[EMAIL PROTECTED] On Behalf Of JOE
CRYSTAL
Sent: Monday, April 14, 2008 5:10 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] Fwd: [ccp4bb] crystallisation robot
Hi,
Does anyone have information about how long it takes to set up a 96-well
tray for the crystallization robots
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