Hi, thank you for you reply. Could you tell me if you try to dye membrane
protein crystal with detergent?
Thanks.
Y.B.
2010-11-18
yybbll
发件人： Matthew Bratkowski
发送时间： 2010-11-17 02:58:40
收件人： CCP4BB
抄送：
主题： Re: [ccp4bb] how to optimize small rod-shaped crystals
I like using
...@bio.mls.eng.osaka-u.ac.jp
Subject: Re: [ccp4bb] how to optimize small rod-shaped crystals
To: CCP4BB@JISCMAIL.AC.UK
Date: Tuesday, November 16, 2010, 8:19 PM
I strongly agree with Eric Larson’s suggestion on trying to see the diffraction
of your crystal. The most straightforward solution. Other
Hi Yibin,
I cannot tell the actual scale of your crystals but judging by the curve of the
drop outline, these crystals look to be plenty big enough to mount in a loop to
stick in the beam and test diffraction. If indeed they are too small to mount a
single crystal, and you are mainly
Hi Yibin,
MiTeGen offers different loops that make it easier to pick really tiny crystals
which can be diffracted at the synchrotron.
If you would like to optimize small needles or rod shaped crystals, you may try
an additive screen.
You may also try to lower the rate of crystal growth.
I like using Izit dye from Hampton (
http://hamptonresearch.com/product_detail.aspx?cid=4sid=41pid=33) to check
if crystals are protein or salt. If the crystals are protein, the dye
should absorb rather readily into the crystals and turn them blue, while the
rest of the drop will eventually turn
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] how to optimize small rod-shaped crystals
I like using Izit dye from Hampton
(http://hamptonresearch.com/product_detail.aspx?cid=4
http://hamptonresearch.com/product_detail.aspx?cid=4sid=41pid=33
sid=41pid=33) to check if crystals are protein or salt
lower
temperature, different ratio of protein:crystallant, etc).
Clement
From: yybbll
Sent: Wednesday, November 17, 2010 2:42 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] how to optimize small rod-shaped crystals
Hi, everybody,
I try to crystallize one membrane protein. All crystals were
.
--
*From:* CCP4 bulletin board [mailto:ccp...@jiscmail.ac.uk] *On Behalf Of
*Matthew
Bratkowski
*Sent:* Tuesday, November 16, 2010 7:58 PM
*To:* CCP4BB@JISCMAIL.AC.UK
*Subject:* Re: [ccp4bb] how to optimize small rod-shaped crystals
I like using Izit dye from Hampton (
http
and a negative control with bona fide salt crystals.
From: CCP4 bulletin board [mailto:ccp...@jiscmail.ac.uk] On Behalf Of
Matthew Bratkowski
Sent: Tuesday, November 16, 2010 7:58 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] how to optimize small rod
