[ccp4bb] increase the solubility of proteins

2011-01-19 Thread Jerry McCully
Dear ALL; Recently I am purifying a cell surface receptor by refolding of its inclusion bodies. Actually I can purify some functional monomers of this protein. However, the protein precipitates during the concentration. Interestingly, the precipitates look like crystals but not

Re: [ccp4bb] increase the solubility of proteins

2011-01-19 Thread Bosch, Juergen
If they look like crystals, why don't you shoot them and see if they diffract ? Some people have been lucky with this approach. Jürgen - Jürgen Bosch Johns Hopkins Bloomberg School of Public Health Department of Biochemistry Molecular Biology Johns Hopkins Malaria Research Institute 615 North