If cost is an issue, I recommend preparing your own MINI Dialysis
Devices at 99.74% savings.
Cut a hole in the lid of an Eppendorf tube, put your sample in the tube,
then close the lid over a small piece of dialysis tubing, trapping it as
a cover. Float inverted in dialysis buffer and Bob's
For even smaller volumes, make a dialysis button from an eppendorf tube:
With a razor blade, cut the tube off leaving only the rim
that the cap snaps into.
Put your sample in the depression in the middle of the cap (inside).
cover with membrane and snap the rim down over it.
(I have done this but
Hi all,
I use Slide-A-Lyzer MINI Dialysis Devices from Thermo for dialysis of small
protein volumes. Are they reusable? if so how can we store them??
Thanks and Regards,
Manjula R
Research Scholar
Department of Biophysics
National Institute of Mental Health and Neurosciences
Bengaluru-29,
Hi Manjula,
I imagine that they could be reusable, if you stored them in a buffer with
some sort of preservative like sodium azide (NB Toxic) or maybe a
bacteriostatic agent like EDTA or something.
However, when you use them, a small amount of protein is going to remain in
the cassette after
