Re: [ccp4bb] mammalian expression vector

2012-03-14 Thread A. Radu Aricescu
CCP4BB@JISCMAIL.AC.UK (on behalf of Jerry McCully for-crystallizai...@hotmail.com) Subject: [ccp4bb] mammalian expression vector To: CCP4BB@JISCMAIL.AC.UK Dear ALL; As an alternative strategy to avoid endotoxin, I plan to express the protein in mammalian cells

[ccp4bb] mammalian expression vector

2012-03-13 Thread Jerry McCully
Dear ALL; As an alternative strategy to avoid endotoxin, I plan to express the protein in mammalian cells. As suggested by others, the typical vector is pcDNA3.1(+). Does anyone have comments on this vector or recommend some other powerful vectors? I am new to mammalian