Self rotation is not very clear - and the top peaks are almost certainly
generated partly by the high crystallographic symmmetry.
Things to check:
1) native Patterson - is there a non-crystallographic translation?
ctruncate checks this for you.
2) twinning - if there is a non-crystallographic
Hi Frank,
Off from the original topic but important to clarify. If I misled the
concepts, I apologize.
Outer shell Rmerge will always be very high:
--
True! Especially when I/Sig ~ 1 or less.
Only I/sigI (and completeness, although it's related) is really
relevant for deciding
Dear Wei,
The unit-cell dimensions are certainly not excessive, we recently published the
2.8A 27kDa uridylate kinase structure from B. anthracis whose crystals were
P6122, a=b=87, c=384 with three UK in the ASU.
As you are apparently not seeing any self rotation peaks, have you looked for a
t
I'm not clear what is the reasoning here behind using a low res cutoff for the
SRF (i.e. lower than the data limit). It seems to me that using all valid data
available can only increase the signal/noise ratio, and omitting good data can
only have a deleterious effect (as will any kind of data i
Um, can't resist here (although not directly relevant to the original
question):
1) Your dataset has a high overall Rmerge. The outmost shell (70%) is
very high, which suggests a need to shrink resolution. What about
I/s(I), redundancy and completeness? Also, how many
reflections (percentage
Dear Perrakis,
The R merge of the lowest resolution shell is 0.08.
The matthews' analysisi is below:
For estimated molecular weight 78750.
Nmol/asym Matthews Coeff %solvent P(2.90) P(tot)
___
1 7.9084.45 0.00 0.00
2
Hi all -
On 15 Jul 2009, at 7:03, Lijun Liu wrote:
Hello dear Wei,
1) Your dataset has a high overall Rmerge. The outmost shell (70%)
is very high, which suggests a need to shrink resolution. What
about I/s(I), redundancy and completeness? Also, how many
reflections (percentage) have
Hello dear Wei,
1) Your dataset has a high overall Rmerge. The outmost shell (70%) is
very high, which suggests a need to shrink resolution. What about I/
s(I), redundancy and completeness? Also, how many reflections
(percentage) have been subjected to rejection? Too many rejections
ma
Dears,
I am sorry for not saying it clearly.
By looking at the 00l lines and by Phenix.xtrige, it is probable P6122 or
P6522. In the molecular replacement searching, I have set to search all the
possible of space group of P622 in Phaser and Molrep. But all the trials
failed.
My model is the structu
Are you sure you're using the right space group? For example, what does
phenix.xtriage suggest for your space group?
Ho
Confometrx
Dears,
I am doing molecular replacement of a protein complex with a P622 data set
with large cell parameters (a=b=135, c=480). The data set seems well. R
merge is 0.17 for all and 0.70 for the last shell of 2.9 angstrom. I am not
sure it is a complex in the crystal. Phenix analysis reveal there is
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