Hi all,
I got one data about 3.0 A, belong to C2 space group. There are two protein
molecules and one 18-nt dsRNA per ASU. The structure of last 100aa
(C-terminal) has been reported, and 400 aa at N-terminalhe has homology
structure with sequence identiy 30%. I try to solve it by MR with phaser.
Hi Lisa,
There are many things you can try and the phaser manual gives a lot of
useful information what to do in difficult cases. From my experience, it
is quite difficult to find solutions for MR with nucleic acids. I
recommend to search only for protein. As a side effect you can use this
the software because
the DNA is denser.
Of course, every case has its own idiosyncracies.
Phoebe
Original message
Date: Tue, 10 Nov 2009 14:19:43 -0500
From: Christian Biertuempfel biertumpf...@niddk.nih.gov
Subject: Re: [ccp4bb] molecular replacement in phaser
To: CCP4BB
