Dear CCP4 members
I have a confusion with molecular replacement. I wish to solve a monomeric
protein in P21 where there are 2 monomers in asymmetric unit. I have a search
model that also has 2 monomers in asymmetric unit.
First I try using Phaser. I use only one chain of the monomer model
in order to rebuild the surface loops in their correct
positions.
HTH,
Fred.
(there are other possibilities of course, such as incorrect space group
assignment)
Message du 15/03/11 07:41
De : Careina Edgooms
A : CCP4BB@JISCMAIL.AC.UK
Copie à :
Objet : [ccp4bb] molecular replacement solution
On 03/15/11 02:41, Careina Edgooms wrote:
Dear CCP4 members
I have a confusion with molecular replacement. I wish to solve a
monomeric protein in P21 where there are 2 monomers in asymmetric unit.
How do you know that? What would the %solvent be with one monomer, and
with two?
If, using
On Mon, 2011-03-14 at 23:41 -0700, Careina Edgooms wrote:
So I am confused. What am I doing wrong? Please help me with
suggestions
Generally, it may be a good idea to post phaser log-file, etc. when
asking a question. You may be able to get more specific suggestions
then - otherwise, all one
Is there NCS? (How's the self rotation look? How about the native
patterson?)
Does your search model have flexible loops? You may have the right
solution, but the flexible loops may be in a different conformation
and cause clashes. Trim the search model to the conserved / structured
