[ccp4bb] off topic question regarding SPR

2013-05-05 Thread Faisal Tarique
Dear all i have a basic question regarding SPR experiment and that is: What is the recommended glycerol concentration in the protein sample for doing SPR experiment and getting the best possible result.? i have kept 10% Glycerol in my protein preparation..is it O.K ? Thanx in advance --

Re: [ccp4bb] off topic question regarding SPR

2013-05-05 Thread Bosch, Juergen
Hi Faisal, whatever makes your protein happy is good. What type of experiment were you thinking of ? Protein-protein interaction or protein-small molecule interaction ? In the latter case add some Tween20 or Brij-35 into the mix. A decent overview can be found under this webpage for what to