P21.. You sure about this space group? (very high confidences for space group
and laue group in pointless?)
F
On Apr 19, 2012, at 12:20 AM, LISA wrote:
> Hi all,
>
> I am trying to solve one structure by molecular replacement with phaser in
> CCP4. This a complex of a multi-domain domains
L.AC.UK>
Subject: [ccp4bb] phaser: high z score but no sol
Hi all,
I am trying to solve one structure by molecular replacement with phaser in
CCP4. This a complex of a multi-domain domains with small ligand. I have
structues of this protein in apo state and with other similar ligand. The
space
, 2012 8:20 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] phaser: high z score but no sol
Hi all,
I am trying to solve one structure by molecular replacement with
phaser in CCP4. This a complex of a multi-domain domains with small
ligand. I
As Randy pointed out, you should check Patterson map for off-origin
peaks. There is also a small chance that you actually have P2 -
systematic absences may result from tNCS nearly colinear with
crystallographic axis.
On Thu, 2012-04-19 at 14:20 +0800, LISA wrote:
> Hi all,
>
> I am trying to sol
Hi,
My first guess would be that this crystal possesses translational NCS, and that
you're using the "old" (distributed with current old CCP4) version of Phaser
that can't handle tNCS. You can tell if this is the case by looking at whether
there's a large off-origin peak in the native Patterso
Hi,
If you have the correct solution, clashes may be due to loops. It may be
an idea to clip these off for the molecular replacement calculations
(loops might be shorter in the structure-to-be-solved than in the search
model, they may have different conformations in the
structure-to-be-solved
Hi all,
I am trying to solve one structure by molecular replacement with phaser in
CCP4. This a complex of a multi-domain domains with small ligand. I have
structues of this protein in apo state and with other similar ligand. The
space group of this crystal is P21. This crystal should have 4 mol
