Hi all,
If anybody is interested in non-viral stable expression, we have a
piggybac-based, doxycycline-inducible system. It is the reference 40 in the
lentiviral paper that Tomas directed to. We’d be happy to distribute the
plasmids.
Zhijie
> On Jan 25, 2020, at 3:26 AM, Tomas Malinauskas
Hi Gloria,
two key papers describing expression (transient and lentivirus-based)
of proteins in HEK293 cells we use to make milligrams of proteins for
crystallization and cryo-EM:
Acta Crystallogr D Biol Crystallogr. 2006 Oct;62(Pt 10):1243-50. Epub
2006 Sep 19.
A time- and cost-efficient system
7:10:06 AM
To: David Briggs ; CCP4BB@JISCMAIL.AC.UK
Subject: RE: [ccp4bb] protein expression in human cells
Dear colleagues,
I wonder if there were a bit less controversial possibility. No matter if that
was less efficient. Would there be an option of using human cell lines that do
not origin f
:49 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] protein expression in human cells
Hi Gloria,
Another vote here for HEK 293 Expi or Freestyle.
The off-the-shelf transfection reagents are super expensive, but I make my own
(happy to share protocols with anyone interested) and we normally get
January 25, 2020 1:31:02 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] protein expression in human cells
In our lab, we see Expi293F works very good.
Thanks,
Reza
Md. Rezaul Karim
Graduate student, PhD Program in Integrated Biomedical Sciences
Morsani College of Medicine
University of South
Hello CCP4-ers,
I was wondering what people have found to be the best human cell line
expression system for making a large quantity of purified recombinant
protein.
Any information and protocols would be greatly appreciated.
Happy 2020, Gloria
