Re: [ccp4bb] protein precipitated when they formed a complex

2007-12-13 Thread Nurit Mirkin
] protein precipitated when they formed a complex To: CCP4BB@JISCMAIL.AC.UK Hi Jerry, have you tried tinkering with the pH? I have had similar situation, where two normally soluble proteins precipitated upon mixing at pH 7.5, my stock buffer. Altering the pH showed that the complex

[ccp4bb] protein precipitated when they formed a complex

2007-12-12 Thread Jerry McCully
Dear All, Recently I posted a question about protein induced protein precipitation. Firstly I'd like to thank many folks for their good ideas. Later on I did a titration experiment with one protein concentration fixed at 0.4mg/ml(about 10uM). Now it is clear that these two

Re: [ccp4bb] protein precipitated when they formed a complex

2007-12-12 Thread Artem Evdokimov
_ From: CCP4 bulletin board [mailto:[EMAIL PROTECTED] On Behalf Of Jerry McCully Sent: Wednesday, December 12, 2007 3:31 PM To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] protein precipitated when they formed a complex Dear All, Recently I posted a question about protein induced