Hi YB,
For membrane protein crystallization it is common practice (although not always
necessary) to dialyze the protein after the final concentration step (against
GF buffer). The problem with DDM is that dialysis is slow due to the low cmc,
and in general it is advisable to finish the prep
Dear Y B Lin,
Measuring detergent has been discussed before on this list - one way is to do
thin-layer-chromatography against a set of standards.
http://www.mail-archive.com/ccp4bb@jiscmail.ac.uk/msg12915.html
A strategy for identification and quantification of
detergents frequently used in
Could anybody tell me how to detect the concentration of detergent?
From Butler et al. (2004) J Mol Biol 340: 797-808
The concentration of DDM was determined by a colorimetric assay that
detects the sugar component of the detergent, which has given results
identical with the standard
Hi YB,
There is a nice new paper online, dealing with this subject:
http://www.ncbi.nlm.nih.gov/pubmed/20850411
Cheers
Phil
Am 04.10.10 17:09 schrieb Owen Pornillos unter o...@scripps.edu:
Could anybody tell me how to detect the concentration of detergent?
From Butler et al. (2004) J Mol
I like the phenol-sulfuric acid colorimetric assay (Anal Biochem. 2005
Jan 1;336(1):117-24). Easy, very linear, no special
ingredients/equipment required. In agreement with their paper, I also
found that DDM passes through the 100 kD concentrator membranes.
Ho
