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From: CCP4 bulletin board [mailto:ccp...@jiscmail.ac.uk] On Behalf Of David
Briggs
Sent: 12 August 2010 20:22
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] Suggestions for Reducing Protein Precipitation
Hi Matt,
Have you tried changing the pH? Is it possible that at pH 8 you are at
the pI
Hi Matt,
Check out the following paper and some screens available commercially based on
these:
Acta Crystallogr D Biol Crystallogr. 2004 Sep;60(Pt 9):1670-3. Epub 2004 Aug 26.
Optimum solubility (OS) screening: an efficient method to optimize buffer
conditions for homogeneity and
Hi Matt,
Have you tried changing the pH? Is it possible that at pH 8 you are at
the pI of your protein (i.e. where it has zero net charge and is at
its least soluble) ?
I have also read a paper (can't find the reference right now) where
the authors precipitated their protein by dialysing into
Hi all.
I found that reference. :0)
Acta Crystallogr D Biol Crystallogr. 2006 Jul;62(Pt 7):833-42. Epub 2006 Jun 20.
Assessment of a preliminary solubility screen to improve
crystallization trials: uncoupling crystal condition searches.
Izaac A, Schall CA, Mueser TC.
