Zn is a very electron rich atom so a 2.3 A resolution data set should
be a fine experiment to determine the number of fully occupied metal
sites. It is always hard to be sure about screen shots of density, but
it looks to me that you only have evidence for one zinc here.
In my opinion, it
The geometry is difficult to see from a static image, but it looks like
you possibly have a His(2)Glu(4) coordination environment around what
looks like one likely metal ion, based on the difference density. Zn(II)
is typcially (but not always) tetrahedral with Zn-O/N bond distances of
On Wed, Jan 16, 2013 at 2:53 PM, Roger Rowlett rrowl...@colgate.edu wrote:
When you are a building a metalloenzyme model you should really have some
solid evidence that a metal ion is present by (1) inclusion in the
crystallization medium, (2) direct determination by an analytical technique,
-- Original Message ---
From: Dale Tronrud det...@uoxray.uoregon.edu
To: CCP4BB@JISCMAIL.AC.UK
Sent: Wed, 16 Jan 2013 14:14:50 -0800
Subject: Re: [ccp4bb] how many metal sites
***
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