Re: [ccp4bb] off topic: Alternatives to GE HiTrap columns

You can also try products from Bio-rad. http://www.bio-rad.com/featured/en/nickel-columns-nickel-resin.html Best! -- 卢作焜 南开大学新生物站A202 Lu Zuokun, Ph.D. Candidate College of Life Science, Nankai University At 2017-02-18 04:31:08, "Kevin Jin" wrote: Sorry, the

Re: [ccp4bb] off topic: Alternatives to GE HiTrap columns

Sorry, the company was CloneTech, now it is Takara (not Takeda). You may talk with Dr. Gia Jokhadze, who is the director of protein chemistry department. He would give you more information. You can find his profile on linkedin. He is very nice person. Probably, you can ask some free sample from

Re: [ccp4bb] off topic: Alternatives to GE HiTrap columns

Probably you can try His-Column from Clonetech (Takeda ?). They modified the column packing using nylon membrane, which offered better flow-through than that of traditional resin-gel column. On Wed, Feb 15, 2017 at 8:57 AM, Markus Seeliger wrote: > Dear all, > we

Re: [ccp4bb] off topic: Alternatives to GE HiTrap columns

We used cOmplete Histag column from Roche, 5 ml prepacked. We bought 3-4 prepacked columns and a bottle of 25 ml (cheaper). The nice thing about this Roche pack, if you closed the outlet and let the pump run into the inlet, the cap will slowly detach .. We use this trick to replace the resin

Re: [ccp4bb] off topic: Alternatives to GE HiTrap columns

I'll second those suggestions and also note that empty XK columns from GE regularly show up on eBay for pennies on the dollar. Roch cOmplete is really great in situations where there are chelators around (especially proteins secreted into eukaryotic tissue culture media). It saved me tremendous

Re: [ccp4bb] off topic: Alternatives to GE HiTrap columns

We have had good luck with columns from Essential Life Solutions ( http://www.essential-life.net/). They are easy to work with and hold up under pressure well. Best, David -- David L. Blum, Ph.D. Director, Bioexpression and Fermentation Facility Department of Biochemistry and Molecular

Re: [ccp4bb] off topic: Alternatives to GE HiTrap columns

Well GE sells empty columns (XK for low back-pressure, tricorn for higher back-pressure) that you can fill with the resin you want. They seem expensive but for that kind of things I don't think any option will be real 'savings'. For nickel affinity we have been having good results with Roche's

Re: [ccp4bb] off topic: Alternatives to GE HiTrap columns

Hi Markus, BioRad has columns as well (prepacked and empty), which follow the establiched connector standard. So usable without the need of adapters. Cheers Christian Am 15.02.2017 um 16:57 schrieb Markus Seeliger: Dear all, we are happy users of all that GE offers around their FPLC

Re: [ccp4bb] off topic: Alternatives to GE HiTrap columns

You could try Goldbio.com. I have not used the His-columns from them, but have used the Protein A columns and they work as well as the Protein A from GE. Daniel A Bonsor PhD. Sundberg Lab Institute of Human Virology University of Maryland, Baltimore 725 W Lombard Street N370 Baltimore Maryland