Dear All,
Thank you for your replies:
2 different methods were suggested for the citrate buffer preparation over a
small range of pH:
1. mixing citric acid with sodium citrate salts
2. mixing citric acid with NaOH
we found the latter method easier for our purpose.
The easiest way to produce repeatable conditions is to titrate a stock
solution (say 1M) of citric acid with NaOH to the desired pH and use that
to mix your screen. That's what Hampton does anyway.
If fine sampling pH, you can mix various ratios of pH 3 and 6.5 buffers.
The pH won't be linear
CCP4BB@JISCMAIL.AC.UKmailto:CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] preparation of citrate buffer pH3-6.5
The easiest way to produce repeatable conditions is to titrate a stock solution
(say 1M) of citric acid with NaOH to the desired pH and use that to mix your
screen. That's what Hampton
of citrate buffer pH3-6.5
The easiest way to produce repeatable conditions is to titrate a stock
solution (say 1M) of citric acid with NaOH to the desired pH and use
that to mix your screen. That's what Hampton does anyway.
If fine sampling pH, you can mix various ratios of pH 3 and 6.5
buffers
it the
concentrations.
http://hamptonresearch.com/make_tray.aspx
Nick
From: Roger Rowlett rrowl...@colgate.edu
Reply-To: Roger Rowlett rrowl...@colgate.edu
Date: Thursday, January 30, 2014 at 7:23 AM
To: CCP4BB@JISCMAIL.AC.UK CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] preparation of citrate
RE citrate buffer preparation
The Calbiochem buffers has some generally useful information about
buffers; pKa and such.
http://www.antibodybeyond.com/books/Calbiochem_Buffers_Booklet_CB0052_E.pdf
http://wolfson.huji.ac.il/purification/PDF/Buffers/Calbiochem_Buffers_Booklet.pdf
--
Reply-To: Roger Rowlett rrowl...@colgate.edu
Date: Thursday, January 30, 2014 at 7:23 AM
To: CCP4BB@JISCMAIL.AC.UK CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] preparation of citrate buffer pH3-6.5
The easiest way to produce repeatable conditions is to titrate a stock
solution (say 1M
-To: Roger Rowlett rrowl...@colgate.edu
mailto:rrowl...@colgate.edu
Date: Thursday, January 30, 2014 at 7:23 AM
To: CCP4BB@JISCMAIL.AC.UK mailto:CCP4BB@JISCMAIL.AC.UK
CCP4BB@JISCMAIL.AC.UK mailto:CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] preparation of citrate
Subject: Re: [ccp4bb] preparation of citrate buffer pH3-6.5
The easiest way to produce repeatable conditions is to titrate a stock
solution (say
1M) of citric acid with NaOH to the desired pH and use that to mix your
screen.
That's what Hampton does anyway.
If fine sampling
