Hi,
I guess Cacodylate is safe as long as you don't come into direct contact
with it, or dispose it off in a careless fashion. Aren't there
substances in biochemical labs which are a equally if not more harmful,
like acryamide or Ethidium Bromide, for instance?
But I agree that it should be
Hi Frank,
I worked with protein purification buffers and crystallization buffers
containing 20mM potassium cacodylate for five years or so. And yes, the
only precaution I used was gloves while weighing the chemical and while
making buffers etc. And not just me, but all of my former colleagues
or 972-8-646-1710
From: CCP4 bulletin board [CCP4BB@JISCMAIL.AC.UK] on behalf of Raji Edayathumangalam
[r...@brandeis.edu]
Sent: Friday, November 09, 2012 3:26 PM
To:
CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] usefulness of cacodylate?
Hi Frank,
I worked with protein purification
Well originally we got these in cacodylate and not much else would diffract.
http://www.pdb.org/pdb/explore/materialsAndMethods.do?structureId=2EPH
But nowadays I have another conditions, which does not require cacodylate and
works well with Hepes.
Jürgen
On Nov 9, 2012, at 7:26 AM, Frank von
Hi Frank,
In our hands, some RNAs only crystallize out of cacodylate buffers. We would
otherwise stop using it out of health and safety concerns.
Blaine
Blaine Mooers
Assistant Professor
Department of Biochemistry and Molecular Biology
University of Oklahoma Health Sciences Center
S.L. Young
Cacodylate, being an arsenic compound, is moderately toxic. You do have
to ingest it, however, for it to be toxic. Normal lab protection should
be sufficient. It is any more concerning to me than lithium salts,
mercury, acrylamide, ethidium bromide, etc.
Having said that, we usually abandon
Math may be frightening but cacodylate seems not...
With a MW of 214 for the trihydrate
a 70 kg clone needs at the 0.5 g/kg LD50 to consume about 35 g of it, which
is 0.16M.
Of a 0.1M solution you'd therefore have to drink 1.6 L or almost 4 pints.
So, prost, cheers, gsuffa, bescheid,
On Fri, Nov 9, 2012 at 7:26 AM, Frank von Delft frank.vonde...@sgc.ox.ac.uk
wrote
Anybody know
a) how hazardous is cacodylate?
b) does it really matter for crystallization screens?
[...]
(We're being subjected to a safety review.)
I know you are in the UK but this wouldn't have
