Hi
I'd guess from the input file that the beam centre may be defined in a
different reference frame to that which Mosflm expects in the image
header; other possibilities are that the distance, wavelength, or
something else (!) is wrong. Indexing is critically dependent on having
the beam
Dear all
I am trying to crystallize a 7kDa protein using MPD as a precipitant at
16C.I have got small florets at 55-65% of MPD in 3-4 days.The problem is
that the drop is precipitating in one day only and the crystals are coming
with precipitation.I have added 5% glycerol and 100mM of Nacl as an
Oh, there is do much you can do!
Lots of alternatives:
1. Decrease or increase the protein concentration (with a corresponding
increase or decrease of precipitant.
2. Use a temperature gradient.
3. Set up in a capillary with liquid-liquid diffusion
4. SEED!
#3 or #4 are your best bets, probably.
Hi all,
thanks a lot for all the responses!
As anticipated, the solution is rather simple - the origin of the beam:
denzo refines to: x=82.6 y=80.3
mosflm reads from header: x=80.0 y=84.0 - bad indexing etc
mosflm works with: x=82.6 y=80.3
No swapping of x and y between denzo and mosflm in this
Hi Eleanor and others:
This is a little too late to follow up on this thread. But here is a
similar question concerning MR and self-rotation.
Without going into the translational part of MR, is it possible to tell
the orientation of a 2-fold axis directly from the cross-rotation peaks?
e.g. I
