I've just now added these as fink packages (cvs unstable). Sorry for
the delay. I dropped the ball.
Steffen Schmidt did the hard work, so many thanks to Seffen!!!
Bill
On Dec 17, 2008, at 7:56 AM, Andrzej Lyskowski wrote:
Hi,
I've just upgraded my fink distro and was wondering what
More specifically, issue
fink selfupdate-cvs (or fink selfupdate-rsync)
fink install itcl itk iwidgets tdom tkimg tktreectrl
On Dec 17, 2008, at 7:56 AM, Andrzej Lyskowski wrote:
Hi,
I've just upgraded my fink distro and was wondering what else has to
be done concerning configuration
Hi
I should say, while this subject has been brought up, that Luke and I
are working hard to remove many of these dependencies, so iMosflm
should work with a more plain vanilla version of TclTk (but you will
still need iTcl and iTk.
On 17 Dec 2008, at 16:34, William G. Scott wrote:
I can only recommend the Motic microscopes. i'm very impressed for $1,70O incl
camera, not as sturdy as Leica (my favorite) but very good image quality.
Drawback: no cold light source. But you can get a gooseneck light and do top
illumination. Do not buy their polarizer, though.
Cheers,
Jens
I would like to draw your attention to the following opening:
Vacancy Reference 3010245
Applications are invited for a Post-doctoral position funded by Cancer
Research UK, to work on structure/function studies of the NMD
machinery in Dr Laura Spagnolo's group. Applicants must hold a PhD in
Dear All,
Does anybody has any comment on using heparin as an additive to
co-crystallise an RNA-binding protein? I am not asking about using it
to form a complex with proteins that are know to bind heparin
specifically, but using it as a general RNA analogue, when we lack the
knowledge of
By the way, is anyone experiencing the following problem with
fink-installed coot?
dyld: Library not loaded: /usr/X11/lib/libXdamage.1.dylib
Referenced from: /sw/bin/coot
Reason: Incompatible library version: coot requires version 3.0.0 or
later, but libXdamage.1.dylib provides version 2.0.0
Hi,
I have some XAS spectra but I'm new in the area. I'm looking for a free
software. I just want to normalize the data and compare between them. Any
suggestion?
Thank you very much.
Eugenio De la Mora
Insituto de Biotecnologia, UNAM.
Yep, this happened because of the latest Mac OS 10.5.6 update. With the
update, my X11 version got downgraded from 2.3.1 to 2.1.5.
Engin
William G. Scott wrote:
Hi Engin:
What do you get in response to the command
otool -L /usr/X11/lib/libXdamage.1.dylib | grep libXdamage.1.dylib
You
Hi Engin:
What do you get in response to the command
otool -L /usr/X11/lib/libXdamage.1.dylib | grep libXdamage.1.dylib
You should see
/usr/X11/lib/libXdamage.1.dylib (compatibility version 3.0.0,
current version 3.0.0)
If you don't, update X11 here (free) to at least 2.3.1:
Hi all-
Just wondering what the consensus is - should a structure refined with
riding hydrogens be deposited with the coordinates for the hydrogens
included? One could argue that, since they were used in the
refinement to obtain the model and statistics reported, they should be
included
Yes, William I do agree with you,
and in the same line of doubt, I have one more thing to ask you.
Where is this .cvspass resides.
I have problem in updating my fink , with fink selfupdate-cvs.
thanks
S.Jayashankar
Research Student
Institute for Biophysical Chemistry
Hannover Medical School
On Wednesday 17 December 2008 15:53:42 Jonathan Winger wrote:
Hi all-
Just wondering what the consensus is - should a structure refined with
riding hydrogens be deposited with the coordinates for the hydrogens
included? One could argue that, since they were used in the
refinement to
Hi Jon,
- obviously, the model should not be manipulated once the final
statistics is obtained, or if it is manipulated, then the final
statistics must be re-calculated;
- I can't imagine someone thinking that hydrogens were refined
individually in a X-ray structure say at 2A resolution.
Hi
After all v6.1.0 did arrive before Christmas :-) Thank you all.
Still I guess Santa, after freezing in the car park, still had to
endure beeing stuck in some chimney, as a consequence of all of us who
wanted to grab a fresh copy, flooding poor CCP4 server. So, after 2
days I
Hello everyone...
I have a protein solubilised in 1% LDAO, 50 mM Phosphate pH 7.5, 150 mM
NaCl. When I mix it with laemmli buffer and heat at 95C, 3 mins for
SDS-PAGE, it aggregates (as in when you mix GuHCl with SDS) and can't be
loaded onto the gel.
Does anyone know why it happens? Thanks a
Is your phosphate a Na-K phosphate? If it is, then you're probably getting a
K salt of SDS which is considerably less soluble than Na or Li salts.
Artem
-Original Message-
From: CCP4 bulletin board [mailto:ccp...@jiscmail.ac.uk] On Behalf Of
Subscribe Ccp4Bb Kn L
Sent: Wednesday,
Besides the K-SDS precipitation it can also be the problem of membrane
proteins generally having a tendency to aggregate when boiled with
SDS. Try to just add the Laemmli buffer and load the gel without
boiling. This is the general procedure for membrane proteins.
Poul
On 18/12/2008, at
Now that everything with coot and imosflm has been settled, how about
sftools? I am getting errors when I try to open an sftools window with
ccp4i, with 6.1.0. I get the same error with a fink-installed mac
version, and a linux installation. Here is the error:
bad window path name
Hi,
you could try without heating. Just vortex your sample a few times after
adding the sample buffer and then load the gel.
good luck,
Daniela
Is your phosphate a Na-K phosphate? If it is, then you're probably getting
a
K salt of SDS which is considerably less soluble than Na or Li
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