Hi Folks,
Has anyone here had any success running a thermofluor (differential
scanning fluorimetry) experiment on a BioRad CFX96 with SYPRO orange as
the fluorophore?
Our local 'technical expert' tells us it should work and gave us some
settings to test, but was very sketchy about any
Hello everyone,
My protein crystallised in the spacegroup P6522 with one protein molecule in
the asymmetric unit. I have a PEG molecule from the crystallization condition
which crosses a two-fold crystallographic symmetry axis. PEG is symmetric hence
this does not violate the crystal symmetry.
Hello Chandrika,
you can either set the occupancy of the molecule to 0.5 and refine it as
a whole molecule or leave the occupancy at 1, refine only one half of the
molecule and let the symmetry operator do the rest.
Chemically the first way makes more sense to me: even though the PEG
Donnie Berkholz wrote:
On 14:57 Tue 28 Apr , Stuart McNicholas wrote:
I clicked the source code link and it didn't send me anywhere. Is the
source code going to show up at some point, or will that wait until the
rewrite is entirely complete?
My apologies, this is now available.
Hi everyone,
Following Chandrika's question, what should I do if one peptide
chain crosses a two-fold crystallographic symmetry axis?
The peptide is not symmetric and the sidechain of one Se-Met (two after CS
operation) is determined and conformed by MAD.
Your sincerely
Hello De-Feng Li,
first of all sorry for changing the subject: I think starting a new thread
from an existing one may hamper people who are going to search the
archives in the future, therefore it is good practice to give it its
separate subject line, even though it certainly is be very
I sometimes notice people using non-siliconized cover glass and it
makes me wonder what pros and cons we can think about using
siliconized glass... any suggestions?
Sang Hoon Joo, PhD
Postdoctoral Associate
Duke University
239 Nanaline H. Duke
Box 3711, DUMC
Durham, NC 27710
Hi Sang Hoon,
When you have a crystal stuck to non-siliconized glass you will
appreciate coated cover slips. The crystal appears to be superglued
onto the glass surface. This can also happen when you transfer a crystal
to another glass plate (e.g. depression plate) and it sinks down to the
bottom.
The siliconized glass will cause the crystallization drop to bead up
more, whereas the untreated glass will cause some mixtures with a low
surface tension to spread like a Bose fluid.
On Apr 29, 2009, at 12:38 PM, Sang Hoon Joo wrote:
I sometimes notice people using non-siliconized cover
Dear Tim Gruene,
But how to illustrate the other one half occupancy of peptide?
Disorder ?
Your sincerely
De-Feng Li
lidef...@moon.ibp.ac.cn
2009-04-30
Defeng Li, Dr.,
Email: lidef...@moon.ibp.ac.cn
National Laboratory of Biomacromolecules,
A post-doctoral position is available in the labs of Drs.Hideaki Moriyama
and Jay F. Storz at the University of Nebraska. The NIH-funded research
project involves an experimental investigation of molecular adaptation.
Specifically, the goal is to identify mechanisms of hemoglobin adaptation to
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