Hi,
You don't say whether you've considered the possibility that the true symmetry
is higher than P61, e.g. P6122. If there's higher symmetry consistent with
your data, then either pointless or xtriage will tell you which space groups to
consider for test refinements. Another good test (if
-
REMINDER: BEAMTIME ON THE ESRF Bio-SAXS BEAMLINE ID14-3
The Bio-SAXS beamline at ID14-3 at the ESRF (
http://www.esrf.fr/UsersAndScience/Experiments/MX/About_our_beamlines/ID14-3
) has now been in operation for over a year.
Robotic sample
I'll update the CCP4 version of the dictionary
(ccp4/lib/data/cif_mm.dic). Thanks for the heads-up.
As you say, the workaround meanwhile is to edit the .cif file to
remove/change any offending lines.
Martyn
On Wed, 2010-03-03 at 11:24 +, Thomas Womack wrote:
I notice that a fair number of
Is this a cause for concern? FOM's are over 0.5 and Phasing Power is over 2.0.
Thanks
FR
-
Francis Reyes M.Sc.
215 UCB
University of Colorado at Boulder
gpg --keyserver pgp.mit.edu --recv-keys 67BA8D5D
8AE2 F2F4 90F7 9640 28BC 686F 78FD 6669 67BA
Hello,
is someone using Nvidia 3D vision + a compatible 1920x1080 23.5 Desktop
Display e.g.
ACER GD245HQ 120 Hz LCD display
OR
Alienware OptX AW2310 120 Hz LCD display?
Is it running nicely with Linux + Nvidia's Linux driver?
How is the stereo quality compared to Zalman's 3D-LCDs or the old
Dear ALL:
Recently we've been trying hard to crystallize a highly glycosylated
protein complex ( 30% percent of carbohydrate in the total 120KD molecular
weight).
IT is a high affinity protein complex. One component can be crystallized in
high salt condition and the other can be
A related question:
Can one use the old Crystal Eyes glasses system with the new LCD displays? And
if not why not?
Peter B.
-Original Message-
From: CCP4 bulletin board [mailto:ccp...@jiscmail.ac.uk] On Behalf Of Christian
Rausch
Sent: 03 March 2010 15:51
To: CCP4BB@JISCMAIL.AC.UK
No, and it has nothing to do with the monitor. It's the sync signal
from the nvidia emitter which isn't compatible with nuvision,
crystaleyes, or edimensional goggles.
On Wed, Mar 3, 2010 at 10:22 AM, Brick, Peter p.br...@imperial.ac.uk wrote:
A related question:
Can one use the old Crystal
I have Nvidia 3D vision running on the Samsung SyncMaster 2233. One note of
caution, for the Quadra FX3800 if you are using dual monitors I haven't found a
way to get TwinView to work with the Nvidia 195.30 beta linux driver. You can
configure for 2 X-screens to drive the two screens as a
Dear all,
we have a protein isolated from mouse liver which crystallized in P1. The
amount of protein was very little so we could not get better crystals. The
protein expressed in E.coli did not yield any usable crystals.
We managed to collect data from 2 crystals after annealing at the
Dear Jerry,
First of all, it will be hard to reproduce the conditions with the
glycosylated protein because by its nature, it is heterogeneous. One
thing I would try with the glycosylated protein is a detergent screen,
or if you don't have one, use a few NDSB's. Second, I would try setting
up
Director, Institute for Structural Biology, Virginia Commonwealth University
(VCU).
VCU seeks an outstanding investigator in the field of structural biology to
direct the VCU Institute for Structural Biology and Drug Discovery (ISBDD),
which was established in 1995 as the nidus of structural
Hi All,
I have a question regarding developing inhibitor for UTP binding protein.
Since UTP is a common nucleotide substrate for a lot of glycoenzymes
similiar to ATP for kinases, developing potent inhibitor for UTP in vitro
may not seem to be an impossible task, or at least it's technically
Hi everyone,
I am trying to model a S-adenosylmethionine (SAM) molecule into the
active site of a protein using the SAH (exists in the crystal structure)
as the template.
What I have already tried but failed so far are
1)Pymol: I loaded the pdbs of SAM and protein-SAH into pymol and copy
Dear all:
I am trying to create a homology model of a coiled-coil for use in molecular
replacement. I have a template poly-ala coiled coil that I like to use, so that
is fine. I want to thread my sequence onto the helix and am trying to find a
server/easy program that will do this. I want to
Hi Yuan,
LSQ within Coot works quite well for superimposing similar ligands. Are you
sure you are selecting the appropriate chain IDs and residue numbers in the LSQ
dialog box? It is a rigid body superposition though so it will only get you in
the neighborhood (i.e. the adenine and sugar
I would echo Ethan on this metric being something of a relic and add a bit
more data.
Several years ago I tried to get a practical solution to the questions:
- when is a refinement finished?
- how to detect the correctable abnormalities (errors) in a structure so they
can all be corrected
Yuan Cheng wrote:
I am trying to model a S-adenosylmethionine (SAM) molecule into the
active site of a protein using the SAH (exists in the crystal structure)
as the template.
2)Coot: I tried to superpose SAM to SAH in coot. Bot SSM superpose and
LSQ superpose didn't work. when I did SSM
I am trying to create a homology model of a coiled-coil for use in
molecular replacement. I have a template poly-ala coiled coil that I like
to use, so that is fine. I want to thread my sequence onto the helix and am
trying to find a server/easy program that will do this.
As a starting point I
Hello,
My aim is to calculate SIRAS/MIRAS phases using PHENIX. Which should be the
correct column labels and associated Differences in the derivative data
one should select to calculate SIRAS phases? I am supplying both native and
derivative data and for the derivative data i am selecting F_HA,
Very easy way is the Swiss-Model server via the alignment mode
Good luck
Rotem
On 4 Mar, 2010, at 0:59, Brett, Thomas wrote:
Dear all:
I am trying to create a homology model of a coiled-coil for use in
molecular replacement. I have a template poly-ala coiled coil that
I like to use, so
Dear Melanie,
To obtain a reasonable error estimate of R-free you could try to refine your
structure against different sets of reflections. You can force the R-free set
to be the reflections flagged with 1 or 2, etc. instead of 0 (at least in
Refmac) and refine to convergence again. You
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